Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)
Key features and details
- Mouse monoclonal [STX01 (HPC-1)] to Syntaxin
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Syntaxin antibody [STX01 (HPC-1)]
See all Syntaxin primary antibodies -
Description
Mouse monoclonal [STX01 (HPC-1)] to Syntaxin -
Host species
Mouse -
Specificity
In hippocampus, the antibody recognizes the stratum radiatum, the layer formed by the fibers of denate granule cells, as they synapse onto hippocampal pyramidal cells. In rat retina, it stains the membrane of the amacrine cell bodies and inner plexiform layer. It recognizes some neurons early in development and can be detected on migrating amacrine cells in embryonic retina. -
Tested Applications & Species
Application Species ICC/IF RatWB Rat -
Immunogen
Recombinant fragment corresponding to Rat Syntaxin aa 3-225.
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Positive control
- IMR-5 cells Cerebral cortex tissue sections. SH-SY5Y cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
STX01 (HPC-1) -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Rat
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WB |
Rat
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Application | Abreviews | Notes |
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WB |
1/100. Detects a band of approximately 31 kDa (predicted molecular weight: 35 kDa).
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ICC/IF |
1/50.
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Notes |
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WB
1/100. Detects a band of approximately 31 kDa (predicted molecular weight: 35 kDa). |
ICC/IF
1/50. |
Target
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Function
Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Post-translational
modificationsPhosphorylated by CK2. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 112755 Human
- Entrez Gene: 2054 Human
- Entrez Gene: 6804 Human
- Entrez Gene: 6809 Human
- Entrez Gene: 116470 Rat
- Entrez Gene: 24923 Rat
- Entrez Gene: 25130 Rat
- Entrez Gene: 81802 Rat
see all -
Alternative names
- EPIM antibody
- Epimorphin antibody
- EPM antibody
see all
Images
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All lanes : Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 3 : Spinal Cord (Rat) Tissue Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
ICC/IF image of ab3265 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab3265 has been referenced in 9 publications.
- Joussain C et al. Botulinum Neurotoxin Light Chains Expressed by Defective Herpes Simplex Virus Type-1 Vectors Cleave SNARE Proteins and Inhibit CGRP Release in Rat Sensory Neurons. Toxins (Basel) 11:N/A (2019). PubMed: 30791373
- Toft-Bertelsen TL et al. Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters. Mol Biol Cell 27:3329-3341 (2016). WB ; Mouse . PubMed: 27605709
- Shen C et al. The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis. Nat Commun 6:8852 (2015). PubMed: 26572858
- Mead B et al. Comparative evaluation of methods for estimating retinal ganglion cell loss in retinal sections and wholemounts. PLoS One 9:e110612 (2014). IHC . PubMed: 25343338
- Yun HJ et al. LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. Exp Mol Med 45:e36 (2013). PubMed: 23949442
- Kunzevitzky NJ et al. Amacrine cell subtypes differ in their intrinsic neurite growth capacity. Invest Ophthalmol Vis Sci 54:7603-13 (2013). IHC ; Rat . PubMed: 24130183
- Kunzevitzky NJ et al. Foxn4 is required for retinal ganglion cell distal axon patterning. Mol Cell Neurosci 46:731-41 (2011). IHC ; Mouse . PubMed: 21334440
- Benagiano V et al. VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex. BMC Neurosci 12:118 (2011). IHC-P ; Rat . PubMed: 22094010
- Kunzevitzky NJ et al. Amacrine cell gene expression and survival signaling: differences from neighboring retinal ganglion cells. Invest Ophthalmol Vis Sci 51:3800-12 (2010). ICC/IF ; Rat . PubMed: 20445109