Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)


  • Product name
    Anti-Syntaxin antibody [STX01 (HPC-1)]
    See all Syntaxin primary antibodies
  • Description
    Mouse monoclonal [STX01 (HPC-1)] to Syntaxin
  • Host species
  • Specificity
    In hippocampus, the antibody recognizes the stratum radiatum, the layer formed by the fibers of denate granule cells, as they synapse onto hippocampal pyramidal cells. In rat retina, it stains the membrane of the amacrine cell bodies and inner plexiform layer. It recognizes some neurons early in development and can be detected on migrating amacrine cells in embryonic retina.
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Rat Syntaxin aa 3-225.

  • Positive control
    • IMR-5 cells Cerebral cortex tissue sections. SH-SY5Y cell lysate



Our Abpromise guarantee covers the use of ab3265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 31 kDa (predicted molecular weight: 35 kDa).
IP 1/200.
ICC/IF 1/50.



  • All lanes : Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Spinal Cord (Rat) Tissue Lysate
    Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa
    Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 1 minute
  • ICC/IF image of ab3265 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Toft-Bertelsen TL  et al. Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters. Mol Biol Cell 27:3329-3341 (2016). WB ; Mouse . Read more (PubMed: 27605709) »
  • Shen C  et al. The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis. Nat Commun 6:8852 (2015). Read more (PubMed: 26572858) »

See all 8 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. I am sorry to hear that you are having problems with the 3 antibodies ab53723, ab11104 and ab3265. Unfortunately, ab53723 has not been tested for IHC-Frozen, horse and pig samples. Also, ab11104 has not yet been teste...

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Thank you very much for your patience. Product ab3265 is raised against Syntaxin 1 of rat origin. Since there are only 2 amino acids that fall within an extracellular region, the chances of Syntaxin 1 (HPC-1) mapping to this region are quite low...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Horse Tissue sections (cranial cervical ganglion)
cranial cervical ganglion
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH Vector labs H-3301

Abcam user community

Verified customer

Submitted Feb 09 2011

Thank you for getting back to me. The details of the epitope region (extracellular) are provided by the source of this antibody and is shown on the datasheet. Please see the link below. I hope this information helps. Please do not hesitate to c...

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Thank you for your enquiry. I am sorry but the epitope sequence ab3265 remains proprietary. I can however tell you that the epitope resides in the extracellular domain of the protein. I hope this information helps. Please do not hesitate to con...

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Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please le...

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