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Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (9)

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Western blot - Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)
  • Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)

Key features and details

  • Mouse monoclonal [STX01 (HPC-1)] to Syntaxin
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

You may also be interested in

Primary
Product image
Anti-SNAP25 antibody [EP3274] (ab108990)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Primary
Product image
Anti-Syntaxin 1a antibody (ab41453)

View more associated products

Overview

  • Product name

    Anti-Syntaxin antibody [STX01 (HPC-1)]
    See all Syntaxin primary antibodies
  • Description

    Mouse monoclonal [STX01 (HPC-1)] to Syntaxin
  • Host species

    Mouse
  • Specificity

    In hippocampus, the antibody recognizes the stratum radiatum, the layer formed by the fibers of denate granule cells, as they synapse onto hippocampal pyramidal cells. In rat retina, it stains the membrane of the amacrine cell bodies and inner plexiform layer. It recognizes some neurons early in development and can be detected on migrating amacrine cells in embryonic retina.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    WB
    Rat
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Rat Syntaxin aa 3-225.

  • Positive control

    • IMR-5 cells Cerebral cortex tissue sections. SH-SY5Y cell lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    STX01 (HPC-1)
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Rat
WB
Rat
Application Abreviews Notes
WB
1/100. Detects a band of approximately 31 kDa (predicted molecular weight: 35 kDa).
ICC/IF
1/50.
Notes
WB
1/100. Detects a band of approximately 31 kDa (predicted molecular weight: 35 kDa).
ICC/IF
1/50.

Target

  • Function

    Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
  • Sequence similarities

    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Post-translational
    modifications

    Phosphorylated by CK2.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P61266 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 112755 Human
    • Entrez Gene: 2054 Human
    • Entrez Gene: 6804 Human
    • Entrez Gene: 6809 Human
    • Entrez Gene: 116470 Rat
    • Entrez Gene: 24923 Rat
    • Entrez Gene: 25130 Rat
    • Entrez Gene: 81802 Rat
    • Omim: 132350 Human
    • Omim: 186590 Human
    • Omim: 600876 Human
    • Omim: 601485 Human
    • SwissProt: P32856 Human
    • SwissProt: P61266 Human
    • SwissProt: Q13277 Human
    • SwissProt: Q16623 Human
    • SwissProt: P32851 Rat
    • SwissProt: P50279 Rat
    • SwissProt: P61265 Rat
    • SwissProt: Q08849 Rat
    • Unigene: 542230 Human
    • Unigene: 98793 Rat
    see all
  • Alternative names

    • EPIM antibody
    • Epimorphin antibody
    • EPM antibody
    • FP17469 antibody
    • HPC 1 antibody
    • neuron-specific antigen HPC 1 antibody
    • P35 1 antibody
    • STX1A antibody
    • Stx1b antibody
    • STX1B_HUMAN antibody
    • STX1B1 antibody
    • STX1B2 antibody
    • STX2 antibody
    • STX2A antibody
    • STX2B antibody
    • STX2C antibody
    • STX3 antibody
    • STX3A antibody
    • Syntaxin 1A brain antibody
    • Syntaxin 1B antibody
    • Syntaxin 1B1 antibody
    • Syntaxin 1B2 antibody
    • Syntaxin 2 antibody
    • Syntaxin 3 antibody
    • Syntaxin 3A antibody
    • Syntaxin-1B antibody
    • Syntaxin-1B1 antibody
    • Syntaxin-1B2 antibody
    see all

Images

  • Western blot - Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)
    Western blot - Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)
    All lanes : Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Spinal Cord (Rat) Tissue Lysate
    Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa
    Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)
    Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)
    ICC/IF image of ab3265 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (9)

Publishing research using ab3265? Please let us know so that we can cite the reference in this datasheet.

ab3265 has been referenced in 9 publications.

  • Joussain C  et al. Botulinum Neurotoxin Light Chains Expressed by Defective Herpes Simplex Virus Type-1 Vectors Cleave SNARE Proteins and Inhibit CGRP Release in Rat Sensory Neurons. Toxins (Basel) 11:N/A (2019). PubMed: 30791373
  • Toft-Bertelsen TL  et al. Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters. Mol Biol Cell 27:3329-3341 (2016). WB ; Mouse . PubMed: 27605709
  • Shen C  et al. The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis. Nat Commun 6:8852 (2015). PubMed: 26572858
  • Mead B  et al. Comparative evaluation of methods for estimating retinal ganglion cell loss in retinal sections and wholemounts. PLoS One 9:e110612 (2014). IHC . PubMed: 25343338
  • Yun HJ  et al. LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. Exp Mol Med 45:e36 (2013). PubMed: 23949442
  • Kunzevitzky NJ  et al. Amacrine cell subtypes differ in their intrinsic neurite growth capacity. Invest Ophthalmol Vis Sci 54:7603-13 (2013). IHC ; Rat . PubMed: 24130183
  • Kunzevitzky NJ  et al. Foxn4 is required for retinal ganglion cell distal axon patterning. Mol Cell Neurosci 46:731-41 (2011). IHC ; Mouse . PubMed: 21334440
  • Benagiano V  et al. VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex. BMC Neurosci 12:118 (2011). IHC-P ; Rat . PubMed: 22094010
  • Kunzevitzky NJ  et al. Amacrine cell gene expression and survival signaling: differences from neighboring retinal ganglion cells. Invest Ophthalmol Vis Sci 51:3800-12 (2010). ICC/IF ; Rat . PubMed: 20445109

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Question

I have unfixed, fresh frozen horse muscle and unfixed, fresh frozen pig muscle I would like to label with anti-SNAP 25, anti synaptobrevin and anti syntaxin for ICC/IF to investigate the neuromuscular junction. 6 micron sections on slides. – stored at -80 Which primary and secondary antibodies would you recommend? I have tried: ab53723 rabbit polyclonal anti-SNAP25 ab11104 mouse monoclonal anti-synaptobrevin (VAMP) ab3265 mouse monoclonal anti-syntaxin but have had no success. I was doubtful of my secondary for the above so which would you recommend? The antibodies above were sent to me by colleagues who has successfully used them on paraffin sections with a labelling kit. I routinely use bungarotoxin to identify the junctions.

Read More

Abcam community

Verified customer

Asked on Nov 21 2011

Answer

Thank you for contacting us. I am sorry to hear that you are having problems with the 3 antibodies ab53723, ab11104 and ab3265. Unfortunately, ab53723 has not been tested for IHC-Frozen, horse and pig samples. Also, ab11104 has not yet been tested in IHC-Frozen and horse samples. It is the same situation for ab3265 which has not yet been tested for IHC in general as well as horse and pig samples. (We refer to fresh frozen tissue as IHC, and to cultured cells as ICC/IF). Thus, we do not know if and how well these antibodies would perform for the application/species used. The correct seconday antibodies would have been an anti-mouse secondary for both ab3265 and ab11104, and an anti-rabbit secondary for ab53723. What fluorophore have you been using? What exactly was the problem that you encountered for each antibody? And what antibody dilution, blocking and detection step did you use? Please let me know. Also, it might be advisable to use a fixation step to ensure the proteins are kept in the correct location. Was a permeabilization step included? The crossreactivity of an antibody depends mostly on the sequence homology between the immunogen and the protein sequence of interest. Do you have the horse and pig sequences for all 3 proteins? That way I could check which antibody might be most suitable for horse and pig samples. I checked the antibodies we currently have in the catalog: For Syntaxin, we have 4 other antibodies that have been tested with pig samples: ab113986 (FITC), ab112200 (biotin), ab112198 and ab24731. But none of these have been tested in IHC or with frozen sections or with horse samples. For Synaptobrevin, we have 3 other antibodies that have been tested with pig samples: ab24735, ab18015 and ab18013. Again, none of these have been tested with IHC or horse samples. For SNAP25, we have ab24732 that has been tested with pig samples, ab117083 that has been predicted to react with pig samples. None of these however were tested with horse or IHC-Fr samples. We have also 4 antibodies that were tested in frzen sections (IHC-Fr), but not yet in pig or horse: ab68859, ab72396, ab68858 and ab72394. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount. As for secondary antibodies, the choice depends on the host of the primary antibody (e.g. for a rabbit-raised primary antibody, the secondary shuld be an anti-rabbit) as well as what conjugation you prefer to use or which detection system is available to you (e.g. which wavelength can be detected). I look forward to hear back from you with more details so that I can assist you further.

Read More

Abcam Scientific Support

Answered on Nov 21 2011

Question

Hello,  Can you confirm that Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265) detects syntaxin 1 extracellular domain? I am surprised because according to uniprot, syntaxin-1A has only 2 amino acid in the extracellular space.  Sincerely  

Read More

Abcam community

Verified customer

Asked on Sep 19 2011

Answer

Thank you very much for your patience. Product ab3265 is raised against Syntaxin 1 of rat origin. Since there are only 2 amino acids that fall within an extracellular region, the chances of Syntaxin 1 (HPC-1) mapping to this region are quite low (very low). However, because we do not characterize the exact eptiope of this antibody, we cannot guarantee the exact region the antibody maps to. I have updated the datasheet. I apologize for the inconvenience. I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on Sep 19 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Syntaxin antibody [STX01 (HPC-1)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (cranial cervical ganglion)
Specification
cranial cervical ganglion
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH Vector labs H-3301
Permeabilization
No
Read More

Abcam user community

Verified customer

Submitted Feb 09 2011

Question

Thank you for your e-mail. How did you get the data that the epitope resides in the extracellular domain of the protein? Is there any reference about the epitope resides? Thank you for your help.

Read More

Abcam community

Verified customer

Asked on Oct 12 2005

Answer

Thank you for getting back to me. The details of the epitope region (extracellular) are provided by the source of this antibody and is shown on the datasheet. Please see the link below. I hope this information helps. Please do not hesitate to contact me should you require further assistance.

Read More

Abcam Scientific Support

Answered on Oct 12 2005

Question

What epitope (amino acid sequence) does the syntaxin monoclonal antibody recognize?

Read More

Abcam community

Verified customer

Asked on Oct 12 2005

Answer

Thank you for your enquiry. I am sorry but the epitope sequence ab3265 remains proprietary. I can however tell you that the epitope resides in the extracellular domain of the protein. I hope this information helps. Please do not hesitate to contact me should you require further assistance.

Read More

Abcam Scientific Support

Answered on Oct 12 2005

Question

I need a syntaxin antibody to label amacrine neurons in mouse retina. I would like to know if the ab3265 cross reacts with mouse. thanks.

Read More

Abcam community

Verified customer

Asked on Feb 26 2004

Answer

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

Read More

Abcam Scientific Support

Answered on Feb 26 2004

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