Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-Syntaxin antibody [STX01 (HPC-1)]
See all Syntaxin primary antibodies -
DescriptionMouse monoclonal [STX01 (HPC-1)] to Syntaxin
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Host speciesMouse
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SpecificityIn hippocampus, the antibody recognizes the stratum radiatum, the layer formed by the fibers of denate granule cells, as they synapse onto hippocampal pyramidal cells. In rat retina, it stains the membrane of the amacrine cell bodies and inner plexiform layer. It recognizes some neurons early in development and can be detected on migrating amacrine cells in embryonic retina.
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Tested applicationsSuitable for: WB, IP, ICC/IFmore details
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Species reactivityReacts with: Rat, Human
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Immunogen
Recombinant fragment corresponding to Rat Syntaxin aa 3-225.
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Positive control
- IMR-5 cells Cerebral cortex tissue sections. SH-SY5Y cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Constituent: PBS -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberSTX01 (HPC-1)
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab3265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100. Detects a band of approximately 31 kDa (predicted molecular weight: 35 kDa). | |
| IP | 1/200. | |
| ICC/IF | 1/50. |
Target
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FunctionPotentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
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Sequence similaritiesBelongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Post-translational
modificationsPhosphorylated by CK2. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 112755 Human
- Entrez Gene: 2054 Human
- Entrez Gene: 6804 Human
- Entrez Gene: 6809 Human
- Entrez Gene: 116470 Rat
- Entrez Gene: 24923 Rat
- Entrez Gene: 25130 Rat
- Entrez Gene: 81802 Rat
see all -
Alternative names
- EPIM antibody
- Epimorphin antibody
- EPM antibody
see all
Images
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![Western blot - Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)](https://www.abcam.com/ps/products/3/ab3265/Images/Syntaxin-Primary-antibodies-ab3265-2.jpg)
Western blot - Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)All lanes : Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 3 : Spinal Cord (Rat) Tissue Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
![Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)](https://www.abcam.com/ps/products/3/ab3265/Images/Syntaxin-Primary-antibodies-ab3265-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265)ICC/IF image of ab3265 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Toft-Bertelsen TL et al. Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters. Mol Biol Cell 27:3329-3341 (2016). WB ; Mouse . Read more (PubMed: 27605709) »
- Shen C et al. The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis. Nat Commun 6:8852 (2015). Read more (PubMed: 26572858) »
Customer reviews and Q&As
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