Overview

  • Product name
    Anti-Syntaxin antibody [STX01 (HPC-1)]
    See all Syntaxin primary antibodies
  • Description
    Mouse monoclonal [STX01 (HPC-1)] to Syntaxin
  • Host species
    Mouse
  • Specificity
    In hippocampus, the antibody recognizes the stratum radiatum, the layer formed by the fibers of denate granule cells, as they synapse onto hippocampal pyramidal cells. In rat retina, it stains the membrane of the amacrine cell bodies and inner plexiform layer. It recognizes some neurons early in development and can be detected on migrating amacrine cells in embryonic retina.
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Rat Syntaxin aa 3-225.

  • Positive control
    • IMR-5 cells Cerebral cortex tissue sections. SH-SY5Y cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab3265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 31 kDa (predicted molecular weight: 35 kDa).
IP 1/200.
ICC/IF 1/50.

Target

Images

  • All lanes : Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Spinal Cord (Rat) Tissue Lysate
    Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa
    Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • ICC/IF image of ab3265 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Toft-Bertelsen TL  et al. Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters. Mol Biol Cell 27:3329-3341 (2016). WB ; Mouse . Read more (PubMed: 27605709) »
  • Shen C  et al. The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis. Nat Commun 6:8852 (2015). Read more (PubMed: 26572858) »
See all 8 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (cranial cervical ganglion)
Specification
cranial cervical ganglion
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH Vector labs H-3301
Permeabilization
No

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Verified customer

Submitted Feb 09 2011

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