Overview

  • Product name

  • Description

    Goat polyclonal to Syntenin 2
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise both human isoforms (NP_056500; NP_536737).
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Syntenin 2 aa 280-292 (C terminal).
    Sequence:

    LLHHTMDHSIPDA

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 0.5% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml.

Target

Images

  • ab38745 staining Syntenin in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated rabbit anti-goat IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab38745 (4µg/ml) staining of paraffin embedded Human Liver shows staining of cytoplasm in hepatocytes. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

References

ab38745 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
Sample
Human Tissue sections (Colon)
Specification
Colon
Permeabilization
Yes - Wash Buffer from Dako with Tween
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

Submitted Mar 30 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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