Description

  • Product name

    Synthetic Human Di-ubiquitin (linkage-specific K29) protein
  • Expression system

    Synthetic
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Synthetic
    • Species

      Human
    • Predicted molecular weight

      17 kDa
    • Additional sequence information

      K29-linked di-ubiquitin is a diubiquitin linked by a native isopeptide bond between the C terminal glycine of the distal ubiquitin and the epsilon amino group of lysine 29 on the proximal ubiquitin.

Specifications

Our Abpromise guarantee covers the use of ab178485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

    Functional Studies

    HPLC

  • Form

    Liquid
  • Additional notes

    ab178485 is a diubiquitin linked by a native isopeptide bond between the C-terminal glycine of the distal ubiquitin and the epsilon amino group of lysine 29 on the proximal ubiquitin. The product is generated using chemical ligation.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

General Info

  • Relevance

    Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling
  • Cellular localization

    Cytoplasmic Nuclear

Images

  • Mass spectrometry analysis of ab178485

  • SDS analysis of ab178485

References

ab178485 has not yet been referenced specifically in any publications.

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