Synthetic Human Di-ubiquitin (linkage-specific K6) protein (ab178486)
Key features and details
- Expression system: Synthetic
- Suitable for: MS, SDS-PAGE, HPLC, Functional Studies
Description
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Product name
Synthetic Human Di-ubiquitin (linkage-specific K6) protein -
Expression system
Synthetic -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Synthetic -
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Species
Human -
Predicted molecular weight
17 kDa -
Additional sequence information
Di-ubiquitin linked by a native isopeptide bond between the C-terminal glycine of the distal ubiquitin and the epsilon amino group of lysine 6 on the proximal ubiquitin.
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Specifications
Our Abpromise guarantee covers the use of ab178486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
HPLC
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.32% Tris HCl, 0.88% Sodium chloride, 0.03% EDTA
General Info
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Relevance
Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. -
Cellular localization
Nuclear Cytoplasmic
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab178486 has not yet been referenced specifically in any publications.