Key features and details
- Rabbit polyclonal to Syntrophin alpha 1
- Suitable for: Flow Cyt, IHC-Fr, ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Syntrophin alpha 1 antibody
See all Syntrophin alpha 1 primary antibodies
DescriptionRabbit polyclonal to Syntrophin alpha 1
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Rabbit, Human
Synthetic peptide conjugated to KLH, corresponding to amino acids 191-206 of Mouse Syntrophin alpha 1.
General notesFor continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Our Abpromise guarantee covers the use of ab11187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/2000. Predicted molecular weight: 60 kDa.|
FunctionAdapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-biphosphate.
Tissue specificityHigh expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta.
Involvement in diseaseDefects in SNTA1 are the cause of long QT syndrome type 12 (LQT12) [MIM:612955]. A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.
Sequence similaritiesBelongs to the syntrophin family.
Contains 1 PDZ (DHR) domain.
Contains 2 PH domains.
Contains 1 SU (syntrophin unique) domain.
DomainThe PH 1 domain mediates the oligomerization in a calcium dependent manner, and the association with the phosphatidylinositol 4,5-biphosphate.
The PDZ domain binds to the last three or four amino acids of ion channels and receptor proteins. The association with dystrophin or related proteins probably leaves the PDZ domain available to recruit proteins to the membrane.
The SU domain binds calmodulin in a calcium-dependent manner.
modificationsPhosphorylated by CaM-kinase II. Phosphorylation may inhibit the interaction with DMD.
Cellular localizationCell membrane > sarcolemma. Cell junction. Cytoplasm > cytoskeleton. In skeletal muscle, it localizes at the cytoplasmic side of the sarcolemmal membrane and at neuromuscular junctions.
- Information by UniProt
- 59 kDa dystrophin-associated protein A1 acidic component 1 antibody
- Acidic alpha 1 syntrophin antibody
- Alpha 1 syntrophin antibody
ab11187 has been referenced in 6 publications.
- Hord JM et al. Age-related alterations in the sarcolemmal environment are attenuated by lifelong caloric restriction and voluntary exercise. Exp Gerontol 83:148-57 (2016). PubMed: 27534381
- Eisinger K et al. Evaluation of the specificity of four commercially available antibodies to alpha-syntrophin. Anal Biochem 484:99-101 (2015). PubMed: 26079703
- Anderova M et al. Altered astrocytic swelling in the cortex of a-syntrophin-negative GFAP/EGFP mice. PLoS One 9:e113444 (2014). WB ; Mouse . PubMed: 25426721
- Koo T et al. Delivery of AAV2/9-Microdystrophin Genes Incorporating Helix 1 of the Coiled-Coil Motif in the C-Terminal Domain of Dystrophin Improves Muscle Pathology and Restores the Level of a1-Syntrophin and a-Dystrobrevin in Skeletal Muscles of mdx Mice. Hum Gene Ther : (2011). IHC-Fr ; Mouse . PubMed: 21453126
- Wang B et al. A canine minidystrophin is functional and therapeutic in mdx mice. Gene Ther : (2008). PubMed: 18432277
- Vandebrouck A et al. Regulation of capacitative calcium entries by alpha1-syntrophin: association of TRPC1 with dystrophin complex and the PDZ domain of alpha1-syntrophin. FASEB J 21:608-17 (2007). PubMed: 17202249