Overview

  • Product name
    Anti-Syntrophin alpha 1 antibody
    See all Syntrophin alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to Syntrophin alpha 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 191-206 of Mouse Syntrophin alpha 1.

  • General notes
    For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-Fr 1/1000.
ICC/IF 1/200.
WB 1/2000. Predicted molecular weight: 60 kDa.

Target

  • Function
    Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-biphosphate.
  • Tissue specificity
    High expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta.
  • Involvement in disease
    Defects in SNTA1 are the cause of long QT syndrome type 12 (LQT12) [MIM:612955]. A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.
  • Sequence similarities
    Belongs to the syntrophin family.
    Contains 1 PDZ (DHR) domain.
    Contains 2 PH domains.
    Contains 1 SU (syntrophin unique) domain.
  • Domain
    The PH 1 domain mediates the oligomerization in a calcium dependent manner, and the association with the phosphatidylinositol 4,5-biphosphate.
    The PDZ domain binds to the last three or four amino acids of ion channels and receptor proteins. The association with dystrophin or related proteins probably leaves the PDZ domain available to recruit proteins to the membrane.
    The SU domain binds calmodulin in a calcium-dependent manner.
  • Post-translational
    modifications
    Phosphorylated by CaM-kinase II. Phosphorylation may inhibit the interaction with DMD.
  • Cellular localization
    Cell membrane > sarcolemma. Cell junction. Cytoplasm > cytoskeleton. In skeletal muscle, it localizes at the cytoplasmic side of the sarcolemmal membrane and at neuromuscular junctions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 59 kDa dystrophin-associated protein A1 acidic component 1 antibody
    • Acidic alpha 1 syntrophin antibody
    • Alpha 1 syntrophin antibody
    • Alpha-1-syntrophin antibody
    • dJ1187J4.5 antibody
    • Dystrophin associated protein A1 59kDa acidic component antibody
    • LQT12 antibody
    • OTTHUMP00000030650 antibody
    • Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody
    • SNT1 antibody
    • Snta1 antibody
    • SNTA1_HUMAN antibody
    • Syntrophin 1 antibody
    • Syntrophin-1 antibody
    • TACIP1 antibody
    see all

References

This product has been referenced in:
  • Hord JM  et al. Age-related alterations in the sarcolemmal environment are attenuated by lifelong caloric restriction and voluntary exercise. Exp Gerontol 83:148-57 (2016). Read more (PubMed: 27534381) »
  • Eisinger K  et al. Evaluation of the specificity of four commercially available antibodies to alpha-syntrophin. Anal Biochem 484:99-101 (2015). Read more (PubMed: 26079703) »
See all 6 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Question
Answer

Thank you for contacting us. I am sorry but I did not receive your email until this morning. This order has been delivered already using the address of the original order (xxxx). The attention line was to Receiving/PO#xxxx. FedEx tracking number is xxxxx. Please let me know if you have any problems receiving this order. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement plus a free RabMAb with the order number xxxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Answer

Thank you for your reply.

Neither of these are for the product from December of last year but are much older. If you could find the number for the most recent order of ab11187 it would be very helpful for creating this replacement. Also, if you would like me to include a RabMAb please let me know which.

I look forward to your reply.

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Answer

Thank you for contacting us. I am very happy to to provide this antibody free of charge to you. I see that the original order for the faulty lot may have been placed in December. Could you please confirm this for me and perhaps provide and order number or PO for this? We current are running a RabMAb promotion as well. I would be happy to provide, free of charge, any full size RabMAb rabbit monoclonal antibody with this. If there is a RabMAb which you are interested in please let me know and I will include this. I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information. Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for providing this information.

I am very sorry that this product did not work as expected and wish to apologize for the inconvenience this has caused. In reviewing the protocol details which you have provided I see no reason why the antibody did not work and believe this may be a vial or lot specific issue. I would be very happy to send to you a free of charge replacement antibody from the latest lot. Of course this replacement would also be guaranteed under our Abpromise details of which may be found at the link below. In order to process a replacement could you please provide the Abcam order confirmation number for this product or the PO number and date of purchase?

I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting us.

I am very sorry to hear that you have been having difficulties when using this recent lot in ICC/IF. Sadly, the lot which you have requested, 79335, is not longer in stock. However, I would be very happy to try and to help you with this issue. In order to better understand this issue; would you be able to send to me the protocol details which are used with this product and the troubleshooting steps which have been performed?

This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link:

https://www.abcam.com/index.html?pageconfig=abpromise

I look forward to your reply. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - other (rat spinal cord cytosolic and membranous extract)
Loading amount
40 µg
Specification
rat spinal cord cytosolic and membranous extract
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 21 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (skeletal muscle)
Specification
skeletal muscle
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2%

Dr. Bruno Constantin

Verified customer

Submitted Mar 02 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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