Recombinant Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14828] to Syntrophin alpha 1
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Syntrophin alpha 1 antibody [EPR14828]
See all Syntrophin alpha 1 primary antibodies -
Description
Rabbit monoclonal [EPR14828] to Syntrophin alpha 1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HT29, HepG2, A549, MCF7, Human muscle and NIH 3T3 lysates; Human brain tissue and bladder transitional cell carcinoma tissue; MCF7 and HT29 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14828 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/280.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/250.
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WB |
1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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Notes |
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Flow Cyt (Intra)
1/280. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/250. |
WB
1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
Target
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Function
Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-biphosphate. -
Tissue specificity
High expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta. -
Involvement in disease
Defects in SNTA1 are the cause of long QT syndrome type 12 (LQT12) [MIM:612955]. A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy. -
Sequence similarities
Belongs to the syntrophin family.
Contains 1 PDZ (DHR) domain.
Contains 2 PH domains.
Contains 1 SU (syntrophin unique) domain. -
Domain
The PH 1 domain mediates the oligomerization in a calcium dependent manner, and the association with the phosphatidylinositol 4,5-biphosphate.
The PDZ domain binds to the last three or four amino acids of ion channels and receptor proteins. The association with dystrophin or related proteins probably leaves the PDZ domain available to recruit proteins to the membrane.
The SU domain binds calmodulin in a calcium-dependent manner. -
Post-translational
modificationsPhosphorylated by CaM-kinase II. Phosphorylation may inhibit the interaction with DMD. -
Cellular localization
Cell membrane > sarcolemma. Cell junction. Cytoplasm > cytoskeleton. In skeletal muscle, it localizes at the cytoplasmic side of the sarcolemmal membrane and at neuromuscular junctions. - Information by UniProt
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Database links
- Entrez Gene: 6640 Human
- Entrez Gene: 20648 Mouse
- Omim: 601017 Human
- SwissProt: Q13424 Human
- SwissProt: Q61234 Mouse
- Unigene: 31121 Human
- Unigene: 1541 Mouse
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Alternative names
- 59 kDa dystrophin-associated protein A1 acidic component 1 antibody
- Acidic alpha 1 syntrophin antibody
- Alpha 1 syntrophin antibody
see all
Images
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All lanes : Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution
Lane 1 : HT29 lysate
Lane 2 : HepG2 lysate
Lane 3 : A549 lysate
Lane 4 : MCF7 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa -
Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution + Human muscle lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa -
Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution + NIH 3T3 lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa -
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Syntrophin alpha 1 with ab188873 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedHT29 cells labeling Syntrophin alpha 1with ab188873 at 1/280 dilution (red)compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab188873 has been referenced in 2 publications.
- Ye R et al. ß1 Syntrophin Supports Autophagy Initiation and Protects against Cerulein-Induced Acute Pancreatitis. Am J Pathol 189:813-825 (2019). PubMed: 30653956
- Egorova TV et al. CRISPR/Cas9-generated mouse model of Duchenne muscular dystrophy recapitulating a newly identified large 430 kb deletion in the human DMD gene. Dis Model Mech 12:N/A (2019). PubMed: 31028078