Overview

  • Product name

    Anti-Syntrophin alpha 1 antibody [EPR14828]
    See all Syntrophin alpha 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14828] to Syntrophin alpha 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Syntrophin alpha 1 aa 1-100. The exact sequence is proprietary.
    Database link: Q13424

  • Positive control

    • HT29, HepG2, A549, MCF7, Human muscle and NIH 3T3 lysates; Human brain tissue and bladder transitional cell carcinoma tissue; MCF7 and HT29 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14828
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/280.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

  • Function

    Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-biphosphate.
  • Tissue specificity

    High expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta.
  • Involvement in disease

    Defects in SNTA1 are the cause of long QT syndrome type 12 (LQT12) [MIM:612955]. A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.
  • Sequence similarities

    Belongs to the syntrophin family.
    Contains 1 PDZ (DHR) domain.
    Contains 2 PH domains.
    Contains 1 SU (syntrophin unique) domain.
  • Domain

    The PH 1 domain mediates the oligomerization in a calcium dependent manner, and the association with the phosphatidylinositol 4,5-biphosphate.
    The PDZ domain binds to the last three or four amino acids of ion channels and receptor proteins. The association with dystrophin or related proteins probably leaves the PDZ domain available to recruit proteins to the membrane.
    The SU domain binds calmodulin in a calcium-dependent manner.
  • Post-translational
    modifications

    Phosphorylated by CaM-kinase II. Phosphorylation may inhibit the interaction with DMD.
  • Cellular localization

    Cell membrane > sarcolemma. Cell junction. Cytoplasm > cytoskeleton. In skeletal muscle, it localizes at the cytoplasmic side of the sarcolemmal membrane and at neuromuscular junctions.
  • Information by UniProt
  • Database links

  • Alternative names

    • 59 kDa dystrophin-associated protein A1 acidic component 1 antibody
    • Acidic alpha 1 syntrophin antibody
    • Alpha 1 syntrophin antibody
    • Alpha-1-syntrophin antibody
    • dJ1187J4.5 antibody
    • Dystrophin associated protein A1 59kDa acidic component antibody
    • LQT12 antibody
    • OTTHUMP00000030650 antibody
    • Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody
    • SNT1 antibody
    • Snta1 antibody
    • SNTA1_HUMAN antibody
    • Syntrophin 1 antibody
    • Syntrophin-1 antibody
    • TACIP1 antibody
    see all

Images

  • All lanes : Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution

    Lane 1 : HT29 lysate
    Lane 2 : HepG2 lysate
    Lane 3 : A549 lysate
    Lane 4 : MCF7 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution + Human muscle lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution + NIH 3T3 lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Syntrophin alpha 1 with ab188873 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HT29 cells labeling Syntrophin alpha 1 with ab188873 at 1/280 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

This product has been referenced in:

  • Ye R  et al. ß1 Syntrophin Supports Autophagy Initiation and Protects against Cerulein-Induced Acute Pancreatitis. Am J Pathol 189:813-825 (2019). Read more (PubMed: 30653956) »
  • Egorova TV  et al. CRISPR/Cas9-generated mouse model of Duchenne muscular dystrophy recapitulating a newly identified large 430 kb deletion in the human DMD gene. Dis Model Mech 12:N/A (2019). Read more (PubMed: 31028078) »
See all 2 Publications for this product

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