Anti-Syntrophin antibody [1351] (ab11425)

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Overview

  • Product name
    Anti-Syntrophin antibody [1351]
  • Description
    Mouse monoclonal [1351] to Syntrophin
  • Host species
    Mouse
  • Specificity
    ab11425 is known to be reactive with the alpha 1, beta 1 and beta 2 subunits of syntrophin.
  • Tested applications
    Suitable for: ICC/IF, IP, WB, IHC-Fr, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Dog, Human, Xenopus laevis, Amphibian, Xenopus tropicalis
    Predicted to work with: Fish
  • Immunogen

    Other Immunogen Type corresponding to Syntrophin. Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.

  • Epitope
    ab11425 is directed against an epitope within the PDZ domain of syntrophin.
  • Positive control
    • In Western Blot, this antibody gave a positive signal in human, mouse and rat skeletal muscle tissue lysates.
  • General notes

    ab11425 is seen as the "gold standard" for syntrophin assessment.

Properties

Applications

Our Abpromise guarantee covers the use of ab11425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 0.2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa).
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-bisphosphate.
  • Tissue specificity
    High expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta.
  • Involvement in disease
    Long QT syndrome 12 (LQT12) [MIM:612955]: A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities
    Belongs to the syntrophin family.
    Contains 1 PDZ (DHR) domain.
    Contains 2 PH domains.
    Contains 1 SU (syntrophin unique) domain.
  • Domain
    The PH 1 domain mediates the oligomerization in a calcium dependent manner, and the association with the phosphatidylinositol 4,5-bisphosphate.
    The PDZ domain binds to the last three or four amino acids of ion channels and receptor proteins. The association with dystrophin or related proteins probably leaves the PDZ domain available to recruit proteins to the membrane.
    The SU domain binds calmodulin in a calcium-dependent manner.
  • Post-translational
    modifications
    Phosphorylated by CaM-kinase II. Phosphorylation may inhibit the interaction with DMD.
  • Cellular localization
    Cell membrane > sarcolemma. Cell junction. Cytoplasm > cytoskeleton. In skeletal muscle, it localizes at the cytoplasmic side of the sarcolemmal membrane and at neuromuscular junctions.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 59 kDa dystrophin-associated protein A1 acidic component 1 antibody
    • Alpha-1-syntrophin antibody
    • Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody
    • SNT1 antibody
    • SNT2 antibody
    • SNT3 antibody
    • Snta1 antibody
    • SNTA1_HUMAN antibody
    • SNTB1 antibody
    • SNTB2 antibody
    • Syntrophin alpha 1 antibody
    • Syntrophin beta 1 antibody
    • Syntrophin beta 2 antibody
    • Syntrophin-1 antibody
    • TACIP1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin antibody [1351] (ab11425)
    Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin antibody [1351] (ab11425)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Syntrophin (green) with ab11425 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin antibody [1351] (ab11425)
    Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin antibody [1351] (ab11425)

    Immunocytochemistry/Immunofluorescence analysis of A2058 cells labeling Syntrophin (green) with ab11425 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin antibody [1351] (ab11425)
    Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin antibody [1351] (ab11425)

    Immunocytochemistry/Immunofluorescence analysis of 293 cells labeling Syntrophin (green) with ab11425 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Flow Cytometry - Anti-Syntrophin antibody [1351] (ab11425)
    Flow Cytometry - Anti-Syntrophin antibody [1351] (ab11425)
    Overlay histogram showing SH-SY5Y cells stained with ab11425 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11425, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Western blot - Anti-Syntrophin antibody [1351] (ab11425)
    Western blot - Anti-Syntrophin antibody [1351] (ab11425)
    All lanes : Anti-Syntrophin antibody [1351] (ab11425) at 5 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 26 kDa, 42 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes
  • Immunoprecipitation - Anti-Syntrophin antibody [1351] (ab11425)
    Immunoprecipitation - Anti-Syntrophin antibody [1351] (ab11425)
    Syntrophin was immunoprecipitated using 0.5mg Mouse Skeletal Muscle tissue lysate, 5µg of Mouse monoclonal to Syntrophin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Skeletal Muscle tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab11425.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 58kDa; Syntrophin

References

This product has been referenced in:
  • Hakim CH  et al. A Five-Repeat Micro-Dystrophin Gene Ameliorated Dystrophic Phenotype in the Severe DBA/2J-mdx Model of Duchenne Muscular Dystrophy. Mol Ther Methods Clin Dev 6:216-230 (2017). Read more (PubMed: 28932757) »
  • Wasala NB  et al. Uniform low-level dystrophin expression in the heart partially preserved cardiac function in an aged mouse model of Duchenne cardiomyopathy. J Mol Cell Cardiol 102:45-52 (2017). Read more (PubMed: 27908661) »
See all 21 Publications for this product

Publishing research using ab11425? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-Syntrophin antibody [1351]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - other (spinal cord membranous/cytosolic protein extract)
Loading amount
20 µg
Specification
spinal cord membranous/cytosolic protein extract
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jun 21 2007

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