Overview

  • Product name
  • Description
    Chicken polyclonal to SYT1
  • Host species
    Chicken
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human SYT1 conjugated to keyhole limpet haemocyanin. Shared between the Human (NP_005630, NCBI) and Mouse (NP_033332, NCBI) sequences.

Properties

Applications

Our Abpromise guarantee covers the use of ab133856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/2000 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/1000 - 1/2000. 2% paraformaldehyde-fixed tissues or cells

Target

  • Function
    May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
  • Sequence similarities
    Belongs to the synaptotagmin family.
    Contains 2 C2 domains.
  • Domain
    The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
    The second C2 domain mediates interaction with SV2A and STN2.
  • Cellular localization
    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Cytoplasm. Synaptic vesicles and chromaffin granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781D2042 antibody
    • FLJ42519 antibody
    • p65 antibody
    • SVP65 antibody
    • Synaptotagmin 2 antibody
    • Synaptotagmin I antibody
    • Synaptotagmin II antibody
    • Synaptotagmin-1 antibody
    • SYT antibody
    • Syt1 antibody
    • SYT1_HUMAN antibody
    • Syt2 antibody
    • SytI antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) analysis of e18 mouse cochlear ganglion tissue labelling SYT1 (green) with ab133856. Tissue was fixed with 4.0% paraformaldehyde. Red - Actin (Phalloidin staining).

  • Anti-SYT1 antibody (ab133856) at 1/1000 dilution + Adult mouse (C57 Black) cerebrum

    Predicted band size: 47 kDa

  • Immunohistochemistry (Frozen sections) analysis of e18 mouse cochlea tissue labelling SYT1 (green) with ab133856. Tissue was fixed with 4% paraformaldehyde. Red - rabbit anti-MAP2, Blue - Cochlear hair cell marker.

References

ab133856 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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