Overview

  • Product name
  • Description
    Chicken polyclonal to SYT1
  • Host species
    Chicken
  • Specificity
    ab53484 recognizes Synaptotagmin 1.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Apteronotus leptorhynchus
  • Immunogen

    Synthetic peptide corresponding to SYT1 conjugated to keyhole limpet haemocyanin.

Properties

Applications

Our Abpromise guarantee covers the use of ab53484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes
    ICC/IF: 1/100.
    WB: 1/1000. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
    • Sequence similarities
      Belongs to the synaptotagmin family.
      Contains 2 C2 domains.
    • Domain
      The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
      The second C2 domain mediates interaction with SV2A and STN2.
    • Cellular localization
      Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Cytoplasm. Synaptic vesicles and chromaffin granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp781D2042 antibody
      • FLJ42519 antibody
      • p65 antibody
      • SVP65 antibody
      • Synaptotagmin 2 antibody
      • Synaptotagmin I antibody
      • Synaptotagmin II antibody
      • Synaptotagmin-1 antibody
      • SYT antibody
      • Syt1 antibody
      • SYT1_HUMAN antibody
      • Syt2 antibody
      • SytI antibody
      see all

    Images

    References

    ab53484 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Apteronotus leptorhynchus Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Triton X-100
    Blocking step
    3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount
    50 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Blocking step
    Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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