Anti-SYT1 antibody (ab82414)
Key features and details
- Rabbit polyclonal to SYT1
- Suitable for: WB
- Reacts with: Mouse, Rat, Xenopus laevis
- Isotype: IgG
Overview
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Product name
Anti-SYT1 antibody
See all SYT1 primary antibodies -
Description
Rabbit polyclonal to SYT1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Xenopus laevis -
Immunogen
Synthetic peptide corresponding to Rat SYT1 (N terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- Rat Brain and Xenopus Ovary lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 48 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 48 kDa). |
Target
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Function
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. -
Sequence similarities
Belongs to the synaptotagmin family.
Contains 2 C2 domains. -
Domain
The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with SV2A and STN2. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Cytoplasm. Synaptic vesicles and chromaffin granules. - Information by UniProt
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Database links
- Entrez Gene: 20979 Mouse
- Entrez Gene: 25716 Rat
- SwissProt: P46096 Mouse
- SwissProt: P21707 Rat
- Unigene: 289702 Mouse
- Unigene: 216272 Rat
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Alternative names
- DKFZp781D2042 antibody
- FLJ42519 antibody
- p65 antibody
see all
Images
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Western blot - Anti-SYT1 antibody (ab82414)All lanes : Anti-SYT1 antibody (ab82414) at 1/1000 dilution
Lane 1 : Rat Brain lysate
Lane 2 : Mouse Brain lysate
Lane 3 : Xenopus Ovary lysate
Developed using the ECL technique.
Predicted band size: 48 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab82414 has been referenced in 1 publication.
- Wang F et al. Concurrent administration of thyroxine and donepezil induces plastic changes in the prefrontal cortex of adult hypothyroid rats. Mol Med Rep 16:3233-3241 (2017). PubMed: 28713915