For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    syt2-antibody-ab113545.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmission Secretory Vesicles Other
Share by email

Anti-SYT2 antibody (ab113545)

  • Datasheet
Reviews (6) Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)
  • Western blot - Anti-SYT2 antibody (ab113545)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)

Key features and details

  • Rabbit polyclonal to SYT2
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SYT2 antibody
    See all SYT2 primary antibodies
  • Description

    Rabbit polyclonal to SYT2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Spinal Cord; Cortex; Hippocampus; Cerebellum; Forebrain; Substantia Nigra. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal brain.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab113545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Mouse
WB
Mouse
All applications
Rat
Dog
Human
Pig
Chimpanzee
Macaque monkey
Gorilla
Chinese hamster
Common marmoset
Orangutan
Application Abreviews Notes
IHC-P (6)
Use a concentration of 0.5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).
Notes
IHC-P
Use a concentration of 0.5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).

Target

  • Function

    May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
  • Sequence similarities

    Belongs to the synaptotagmin family.
    Contains 2 C2 domains.
  • Domain

    The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
    The second C2 domain mediates interaction with Stonin 2.
  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Synaptic vesicles and chromaffin granules.
  • Target information above from: UniProt accession Q8N9I0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 127833 Human
    • Entrez Gene: 20980 Mouse
    • Entrez Gene: 24805 Rat
    • Omim: 600104 Human
    • SwissProt: Q8N9I0 Human
    • SwissProt: P46097 Mouse
    • SwissProt: P29101 Rat
    • Unigene: 25422 Human
    • Unigene: 5102 Mouse
    • Unigene: 88574 Rat
    see all
  • Alternative names

    • Synaptotagmin 2 antibody
    • Synaptotagmin II antibody
    • Synaptotagmin-2 antibody
    • Synaptotagmin2 antibody
    • SynaptotagminII antibody
    • SYT 2 antibody
    • Syt II antibody
    • Syt2 antibody
    • SYT2_HUMAN antibody
    • SytII antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)

    IHC-P image of SYT2 staining on sections from rat thalamic nucleus (upper image) and the CA3 region of the rat hippocampus (lower image) using ab113545 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours 10 minutes at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)

    IHC-P image of SYT2 staining on sections from mouse sagittal hippocampus (upper image) and dentate nucleus of the mouse cerebellum (lower image) using ab113545 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)

    IHC-P image of SYT2 staining on Marmoset cerebellum sections using ab113545(1:7000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:7000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG  (1:250). 

    See Abreview

  • Western blot - Anti-SYT2 antibody (ab113545)
    Western blot - Anti-SYT2 antibody (ab113545)
    All lanes : Anti-SYT2 antibody (ab113545) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate
    Lane 3 : Mouse Cortex Tissue Lysate
    Lane 4 : Mouse Hippocampus Tissue Lysate
    Lane 5 : Cerebellum Mouse Tissue Lysate
    Lane 6 : Forebrain (Mouse) Tissue Lysate
    Lane 7 : Mouse Substantia Nigra Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    The predicted molecular weight of SYT2 is 47 kDa (SwissProt), however we expect to observe a banding pattern around 70 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)

    IHC image of SYT2 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113545, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab113545? Please let us know so that we can cite the reference in this datasheet.

    ab113545 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Dog Tissue sections (Cerebellum)
    Specification
    Cerebellum
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 07 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Goat Tissue sections (Cerebellum)
    Specification
    Cerebellum
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 07 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Cat Tissue sections (Cerebellum)
    Specification
    Cerebellum
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 07 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 06 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 06 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SYT2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Marmoset (common) Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted May 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.