Anti-SYT2 antibody (ab113545)

IHC-P image of SYT2 staining on sections from rat thalamic nucleus (upper image) and the CA3 region of the rat hippocampus (lower image) using ab113545 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours 10 minutes at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).

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IHC-P image of SYT2 staining on sections from mouse sagittal hippocampus (upper image) and dentate nucleus of the mouse cerebellum (lower image) using ab113545 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).

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IHC-P image of SYT2 staining on Marmoset cerebellum sections using ab113545(1:7000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:7000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG  (1:250). 

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All lanes : Anti-SYT2 antibody (ab113545) at 1 µg/ml

Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : Cerebellum Mouse Tissue Lysate
Lane 6 : Forebrain (Mouse) Tissue Lysate
Lane 7 : Mouse Substantia Nigra Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?


Exposure time: 2 minutes


The predicted molecular weight of SYT2 is 47 kDa (SwissProt), however we expect to observe a banding pattern around 70 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

IHC image of SYT2 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113545, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.