The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
Use a concentration of 5 µg/ml.
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
Belongs to the synaptotagmin family. Contains 2 C2 domains.
The first C2 domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2.
Immunocytochemistry with SH-SY5Y cells stained with Anti-SYT2 antibody (ab154035) in red and DAPI in blue, as a counter stain. The target protein locates to the plasma membrane (red).
Fixation: 4% Paraformaldehyde, 20 min.
Permeabilization: 0.1% Triton X-100, 10min.
Blocking: 10% Goat Serum/PBS, 1 hour.
Primary: Anti-SYT2 antibody (ab154035) 5 µg/ml in 10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C.
Secondary: Alexa Fluor® 594 Goat Anti-Mouse (H&L) used at a 1/1000 dilution in 10% Goat Serum/PBS, 1hr at RT.
Washing: 3x 1% Goat Serum/PBS, 10 mins/wash
DAPI: 2 µM in 1% Goat Serum/PBS, 10 mins