Product nameAnti-SYT2 antibody [ZNP-1]
See all SYT2 primary antibodies
DescriptionMouse monoclonal [ZNP-1] to SYT2
Tested applicationsSuitable for: IP, WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human, Zebrafish
Does not react with: Cow
Tissue, cells or virus. This information is considered to be commercially sensitive.
- Human, Rat, Mouse and Zebrafish Brain Homogenate Lysate; SH-SY5Y cells. IHC-P: Mouse and rat cerebellum tissue.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: Human serum albumin
Concentration information loading...
Purification notesNear homogeneity as judged by SDS-PAGE. ab154035 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation.
Our Abpromise guarantee covers the use of ab154035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
Sequence similaritiesBelongs to the synaptotagmin family.
Contains 2 C2 domains.
DomainThe first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Synaptic vesicles and chromaffin granules.
- Information by UniProt
- Synaptotagmin 2 antibody
- Synaptotagmin II antibody
- Synaptotagmin-2 antibody
All lanes : Anti-SYT2 antibody [ZNP-1] (ab154035) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Rat Brain Homogenate Lysate
Lane 3 : Mouse Brain Homogenate Lysate
Lane 4 : Zebrafish Brain Homogenate Lysate
Lysates/proteins at 15 µg per lane.
All lanes : Goat polyclonal to Mouse IgG – HRP at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellum tissue sections labeling SYT2 with purified ab154035 at 1/4000 dilution (0.25 µg/mL). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes. Leica mouse (DS9800) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellum tissue sections labeling SYT2 with purified ab154035 at 1/4000 dilution (0.25 µg/mL). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes. Leica mouse (DS9800) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.
Immunocytochemistry with SH-SY5Y cells stained with Anti-SYT2 antibody (ab154035) in red and DAPI in blue, as a counter stain. The target protein locates to the plasma membrane (red).
Fixation: 4% Paraformaldehyde, 20 min.
Permeabilization: 0.1% Triton X-100, 10min.
Blocking: 10% Goat Serum/PBS, 1 hour.
Primary: Anti-SYT2 antibody (ab154035) 5 µg/ml in 10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C.
Secondary: Alexa Fluor® 594 Goat Anti-Mouse (H&L) used at a 1/1000 dilution in 10% Goat Serum/PBS, 1hr at RT.
Washing: 3x 1% Goat Serum/PBS, 10 mins/wash
DAPI: 2 µM in 1% Goat Serum/PBS, 10 mins
ab154035 has not yet been referenced specifically in any publications.