Product nameAnti-SYVN1 antibody [EP7459]
See all SYVN1 primary antibodies
DescriptionRabbit monoclonal [EP7459] to SYVN1
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IPmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SYVN1 aa 150-250 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Database link: Q86TM6
- 293T, HepG2, HeLa, Ramos and SH-SY5Y cell lysates, HeLa and 293T cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 67 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
FunctionActs as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
Tissue specificityUbiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the HRD1 family.
Contains 1 RING-type zinc finger.
DomainThe RING-type zinc finger is required for E3 ligase activity.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 1200010C09Rik antibody
- DER3 antibody
- E3 ubiquitin-protein ligase synoviolin antibody
Immunofluorescence analysis of HeLa cells labeling SYVN1, using ab170901 at a 1/100 dilution (red), and DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling SYVN1 using ab170901 at a 1/10 dilution
All lanes : Anti-SYVN1 antibody [EP7459] (ab170901) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HepG2 cell line lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labeled goat anti-rabbit. at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
Flow cytometry analysis of permeabilized 293T cells labeling SYVN1 (red), using ab170901 at a 1/10 dilution, or a negative control rabbit IgG (green).
This product has been referenced in:
- Feng L et al. Ubiquitin ligase SYVN1/HRD1 facilitates degradation of the SERPINA1 Z variant/a-1-antitrypsin Z variant via SQSTM1/p62-dependent selective autophagy. Autophagy 13:686-702 (2017). Read more (PubMed: 28121484) »
- Kong S et al. Endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 controls B-cell immunity through degradation of the death receptor CD95/Fas. Proc Natl Acad Sci U S A 113:10394-9 (2016). Read more (PubMed: 27573825) »