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  1. Link

    syvn1hrd1-antibody-ab118483.pdf

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Cell Biology Apoptosis Intracellular Survivin / IAPs
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Anti-SYVN1/HRD1 antibody (ab118483)

  • Datasheet
Submit a review Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYVN1/HRD1 antibody (ab118483)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYVN1/HRD1 antibody (ab118483)
  • Western blot - Anti-SYVN1/HRD1 antibody (ab118483)
  • Western blot - Anti-SYVN1/HRD1 antibody (ab118483)

Key features and details

  • Rabbit polyclonal to SYVN1/HRD1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Protein
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Recombinant Human SYVN1/HRD1 protein (ab132449)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-IRE1 (phospho S724) antibody (ab48187)

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Overview

  • Product name

    Anti-SYVN1/HRD1 antibody
    See all SYVN1/HRD1 primary antibodies
  • Description

    Rabbit polyclonal to SYVN1/HRD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human SYVN1/HRD1 (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: NP_115807.1

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.4
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E3 Ubiquitin Ligases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SYVN1/HRD1 protein (ab132449)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab118483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/100 - 1/500. Predicted molecular weight: 67 kDa.
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/100 - 1/500. Predicted molecular weight: 67 kDa.
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the HRD1 family.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger is required for E3 ligase activity.
  • Post-translational
    modifications

    Not N-glycosylated.
    Auto-ubiquitinated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession Q86TM6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 84447 Human
    • Entrez Gene: 74126 Mouse
    • Omim: 608046 Human
    • SwissProt: Q86TM6 Human
    • SwissProt: Q9DBY1 Mouse
    • Unigene: 75859 Human
    • Unigene: 149870 Mouse
    • Alternative names

      • 1200010C09Rik antibody
      • DER3 antibody
      • E3 ubiquitin-protein ligase synoviolin antibody
      • HMG coA reductase degradation 1 homolog antibody
      • HRD1 antibody
      • KIAA1810 antibody
      • MGC40372 antibody
      • OTTHUMP00000230429 antibody
      • OTTHUMP00000230430 antibody
      • OTTHUMP00000230431 antibody
      • OTTHUMP00000230432 antibody
      • Synovial apoptosis inhibitor 1 antibody
      • Synovial apoptosis inhibitor 1, synoviolin antibody
      • Synoviolin 1 antibody
      • Synoviolin 1 isoform b antibody
      • SYNOVIOLIN antibody
      • SYVN1 antibody
      • SYVN1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYVN1/HRD1 antibody (ab118483)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYVN1/HRD1 antibody (ab118483)

      ab118483 at 10&microg/ml staining SYVN1/HRD1 in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYVN1/HRD1 antibody (ab118483)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYVN1/HRD1 antibody (ab118483)

      ab118483 at 10&microg/ml staining SYVN1/HRD1 in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

    • Western blot - Anti-SYVN1/HRD1 antibody (ab118483)
      Western blot - Anti-SYVN1/HRD1 antibody (ab118483)
      Anti-SYVN1/HRD1 antibody (ab118483) at 1/100 dilution + T47D cell lysate at 35 µg

      Predicted band size: 67 kDa

    • Western blot - Anti-SYVN1/HRD1 antibody (ab118483)
      Western blot - Anti-SYVN1/HRD1 antibody (ab118483)
      Anti-SYVN1/HRD1 antibody (ab118483) at 1/100 dilution + Mouse kidney tissue lysate

      Predicted band size: 67 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (3)

    Publishing research using ab118483? Please let us know so that we can cite the reference in this datasheet.

    ab118483 has been referenced in 3 publications.

    • Guo X  et al. HRD1 inhibits fatty acid oxidation and tumorigenesis by ubiquitinating CPT2 in triple-negative breast cancer. Mol Oncol 15:642-656 (2021). PubMed: 33207079
    • Fan Y  et al. CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. Mol Cancer 20:25 (2021). PubMed: 33530981
    • Cheng X  et al. ATRA protects skin fibroblasts against UV-induced oxidative damage through inhibition of E3 ligase Hrd1. Mol Med Rep 20:2294-2302 (2019). PubMed: 31322186

    Customer reviews and Q&As

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