Anti-SYVN1/HRD1 antibody (ab118483)
Key features and details
- Rabbit polyclonal to SYVN1/HRD1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SYVN1/HRD1 antibody
See all SYVN1/HRD1 primary antibodies -
Description
Rabbit polyclonal to SYVN1/HRD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human SYVN1/HRD1 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: NP_115807.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/100 - 1/500. Predicted molecular weight: 67 kDa.
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| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/100 - 1/500. Predicted molecular weight: 67 kDa. |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. -
Tissue specificity
Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level). -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the HRD1 family.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger is required for E3 ligase activity. -
Post-translational
modificationsNot N-glycosylated.
Auto-ubiquitinated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 84447 Human
- Entrez Gene: 74126 Mouse
- Omim: 608046 Human
- SwissProt: Q86TM6 Human
- SwissProt: Q9DBY1 Mouse
- Unigene: 75859 Human
- Unigene: 149870 Mouse
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Alternative names
- 1200010C09Rik antibody
- DER3 antibody
- E3 ubiquitin-protein ligase synoviolin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYVN1/HRD1 antibody (ab118483)
ab118483 at 10µg/ml staining SYVN1/HRD1 in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYVN1/HRD1 antibody (ab118483)ab118483 at 10µg/ml staining SYVN1/HRD1 in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Western blot - Anti-SYVN1/HRD1 antibody (ab118483)Anti-SYVN1/HRD1 antibody (ab118483) at 1/100 dilution + T47D cell lysate at 35 µg
Predicted band size: 67 kDa -
Western blot - Anti-SYVN1/HRD1 antibody (ab118483)Anti-SYVN1/HRD1 antibody (ab118483) at 1/100 dilution + Mouse kidney tissue lysate
Predicted band size: 67 kDa
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab118483 has been referenced in 3 publications.
- Guo X et al. HRD1 inhibits fatty acid oxidation and tumorigenesis by ubiquitinating CPT2 in triple-negative breast cancer. Mol Oncol 15:642-656 (2021). PubMed: 33207079
- Fan Y et al. CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. Mol Cancer 20:25 (2021). PubMed: 33530981
- Cheng X et al. ATRA protects skin fibroblasts against UV-induced oxidative damage through inhibition of E3 ligase Hrd1. Mol Med Rep 20:2294-2302 (2019). PubMed: 31322186
