• Product name

  • Description

    Rabbit polyclonal to SYVN1/HRD1
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human SYVN1/HRD1 aa 567-617. The exact sequence is proprietary. (NP_757385.1).
    Database link: Q86TM6

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as SYVN1




Our Abpromise guarantee covers the use of ab225891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 67 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function

    Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the HRD1 family.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger is required for E3 ligase activity.
  • Post-translational

    Not N-glycosylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1200010C09Rik antibody
    • DER3 antibody
    • E3 ubiquitin-protein ligase synoviolin antibody
    • HMG coA reductase degradation 1 homolog antibody
    • HRD1 antibody
    • KIAA1810 antibody
    • MGC40372 antibody
    • OTTHUMP00000230429 antibody
    • OTTHUMP00000230430 antibody
    • OTTHUMP00000230431 antibody
    • OTTHUMP00000230432 antibody
    • Synovial apoptosis inhibitor 1 antibody
    • Synovial apoptosis inhibitor 1, synoviolin antibody
    • Synoviolin 1 antibody
    • Synoviolin 1 isoform b antibody
    • SYNOVIOLIN antibody
    • SYVN1 antibody
    • SYVN1_HUMAN antibody
    see all


  • All lanes : Anti-SYVN1/HRD1 antibody (ab225891) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 67 kDa

    Exposure time: 30 seconds
  • SYVN1/HRD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225891 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225891 at 1 µg/ml.

    Lane 1: ab225891 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 seconds.


ab225891 has not yet been referenced specifically in any publications.

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