Recombinant Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7459] to SYVN1/HRD1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SYVN1/HRD1 antibody [EP7459]
See all SYVN1/HRD1 primary antibodies -
Description
Rabbit monoclonal [EP7459] to SYVN1/HRD1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SYVN1/HRD1 aa 150-250 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Database link: Q86TM6 -
Positive control
- 293T, HepG2, HeLa, Ramos and SH-SY5Y cell lysates, HeLa and 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP7459 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
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ICC/IF |
1/100 - 1/250.
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IP |
1/10 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 67 kDa. |
ICC/IF
1/100 - 1/250. |
IP
1/10 - 1/100. |
Target
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Function
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. -
Tissue specificity
Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level). -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the HRD1 family.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger is required for E3 ligase activity. -
Post-translational
modificationsNot N-glycosylated.
Auto-ubiquitinated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 84447 Human
- Entrez Gene: 74126 Mouse
- Entrez Gene: 361712 Rat
- Omim: 608046 Human
- SwissProt: Q86TM6 Human
- SwissProt: Q9DBY1 Mouse
- Unigene: 75859 Human
- Unigene: 149870 Mouse
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Alternative names
- 1200010C09Rik antibody
- DER3 antibody
- E3 ubiquitin-protein ligase synoviolin antibody
see all
Images
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All lanes : Anti-SYVN1/HRD1 antibody [EP7459] (ab170901) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa -
Immunofluorescence analysis of HeLa cells labeling SYVN1/HRD1, using ab170901 at a 1/100 dilution (red), and DAPI nuclear staining (blue).
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Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling SYVN1/HRD1 using ab170901 at a 1/10 dilution
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Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling SYVN1/HRD1 (red), using ab170901 at a 1/10 dilution, or a negative control rabbit IgG (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab170901 has been referenced in 16 publications.
- Shi Y et al. E3 ubiquitin ligase SYVN1 is a key positive regulator for GSDMD-mediated pyroptosis. Cell Death Dis 13:106 (2022). PubMed: 35115505
- Sun L et al. The E3 Ubiquitin Ligase SYVN1 Plays an Antiapoptotic Role in Polycystic Ovary Syndrome by Regulating Mitochondrial Fission. Oxid Med Cell Longev 2022:3639302 (2022). PubMed: 36193086
- Ma C et al. miR-602 Activates NRF2 Antioxidant Pathways to Protect HBMECs from OGD/R-Induced Oxidative Stress via Targeting KEAP1 and HRD1. Dis Markers 2022:6967573 (2022). PubMed: 36193504
- Tian JH et al. Zonisamide, an antiepileptic drug, alleviates diabetic cardiomyopathy by inhibiting endoplasmic reticulum stress. Acta Pharmacol Sin 42:393-403 (2021). PubMed: 32647341
- Wu Q et al. Zonisamide alleviates cardiac hypertrophy in rats by increasing Hrd1 expression and inhibiting endoplasmic reticulum stress. Acta Pharmacol Sin 42:1587-1597 (2021). PubMed: 33495518