Recombinant Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
![Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.com/ps/products/170/ab170901/Images/ab170901-172098-anti-syvn1-antibody-ep7459-western-blot.jpg "Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7459] to SYVN1/HRD1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SYVN1/HRD1 antibody [EP7459]
See all SYVN1/HRD1 primary antibodies -
Description
Rabbit monoclonal [EP7459] to SYVN1/HRD1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human SYVN1/HRD1 aa 150-250 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Database link: Q86TM6 -
Positive control
- 293T, HepG2, HeLa, Ramos and SH-SY5Y cell lysates, HeLa and 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP7459 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
|
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| ICC/IF |
1/100 - 1/250.
|
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| IP |
1/10 - 1/100.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 67 kDa. |
|
ICC/IF
1/100 - 1/250. |
|
IP
1/10 - 1/100. |
Application notes
Is unsuitable for IHC-P.
Target
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Function
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. -
Tissue specificity
Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level). -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the HRD1 family.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger is required for E3 ligase activity. -
Post-translational
modificationsNot N-glycosylated.
Auto-ubiquitinated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 84447 Human
- Entrez Gene: 74126 Mouse
- Entrez Gene: 361712 Rat
- Omim: 608046 Human
- SwissProt: Q86TM6 Human
- SwissProt: Q9DBY1 Mouse
- Unigene: 75859 Human
- Unigene: 149870 Mouse
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Alternative names
- 1200010C09Rik antibody
- DER3 antibody
- E3 ubiquitin-protein ligase synoviolin antibody
see all
Images
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Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)All lanes : Anti-SYVN1/HRD1 antibody [EP7459] (ab170901) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HepG2 cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit. at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.com/ps/products/170/ab170901/Images/ab170901-172100-anti-syvn1-antibody-ep7459-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)Immunofluorescence analysis of HeLa cells labeling SYVN1/HRD1, using ab170901 at a 1/100 dilution (red), and DAPI nuclear staining (blue).
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![Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.com/ps/products/170/ab170901/Images/ab170901-172099-anti-syvn1-antibody-ep7459-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling SYVN1/HRD1 using ab170901 at a 1/10 dilution
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![Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.com/ps/products/170/ab170901/Images/ab170901-172097-anti-syvn1-antibody-ep7459-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling SYVN1/HRD1 (red), using ab170901 at a 1/10 dilution, or a negative control rabbit IgG (green).
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![Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.com/ps/products/170/ab170901/Images/ab170901-5-benefits-of-recombinant-antibodies.png)
Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab170901 has been referenced in 10 publications.
- Liu S et al. TRIM27 acts as an oncogene and regulates cell proliferation and metastasis in non-small cell lung cancer through SIX3-ß-catenin signaling. Aging (Albany NY) 12:25564-25580 (2020). PubMed: 33264103
- He YX et al. Zonisamide Ameliorates Cognitive Impairment by Inhibiting ER Stress in a Mouse Model of Type 2 Diabetes Mellitus. Front Aging Neurosci 12:192 (2020). PubMed: 32754028
- Jin Y et al. The role of Hrd1 in ultraviolet (UV) radiation induced photoaging. Aging (Albany NY) 12:21273-21289 (2020). PubMed: 33168784
- Xu BY et al. Rifampicin induces clathrin-dependent endocytosis and ubiquitin-proteasome degradation of MRP2 via oxidative stress-activated PKC-ERK/JNK/p38 and PI3K signaling pathways in HepG2 cells. Acta Pharmacol Sin N/A:N/A (2019). PubMed: 31316180
- Cheng X et al. ATRA protects skin fibroblasts against UV-induced oxidative damage through inhibition of E3 ligase Hrd1. Mol Med Rep 20:2294-2302 (2019). PubMed: 31322186
- Bushell SR et al. The structural basis of lipid scrambling and inactivation in the endoplasmic reticulum scramblase TMEM16K. Nat Commun 10:3956 (2019). PubMed: 31477691
- Xu ZM et al. Ginsenoside Rg1 Prevents Doxorubicin-Induced Cardiotoxicity through the Inhibition of Autophagy and Endoplasmic Reticulum Stress in Mice. Int J Mol Sci 19:N/A (2018). PubMed: 30463294
- Feng L et al. Ubiquitin ligase SYVN1/HRD1 facilitates degradation of the SERPINA1 Z variant/a-1-antitrypsin Z variant via SQSTM1/p62-dependent selective autophagy. Autophagy 13:686-702 (2017). PubMed: 28121484
- Kong S et al. Endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 controls B-cell immunity through degradation of the death receptor CD95/Fas. Proc Natl Acad Sci U S A 113:10394-9 (2016). PubMed: 27573825
- Shen H et al. Endoplasmic Reticulum Stress Induces the Early Appearance of Pro-apoptotic and Anti-apoptotic Proteins in Neurons of Five Familial Alzheimer's Disease Mice. Chin Med J (Engl) 129:2845-2852 (2016). PubMed: 27901000