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    syvn1hrd1-antibody-ep7459-ab170901.pdf

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Cell Biology Apoptosis Intracellular Survivin / IAPs
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RecombinantRabMAb

Recombinant Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)

  • Datasheet
  • SDS
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Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
  • Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
  • Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
  • Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
  • Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP7459] to SYVN1/HRD1
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-SYVN1/HRD1 antibody [EP7459]
    See all SYVN1/HRD1 primary antibodies
  • Description

    Rabbit monoclonal [EP7459] to SYVN1/HRD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human SYVN1/HRD1 aa 150-250 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q86TM6

  • Positive control

    • 293T, HepG2, HeLa, Ramos and SH-SY5Y cell lysates, HeLa and 293T cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP7459
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E3 Ubiquitin Ligases

Associated products

  • Alternative Versions

    • PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)
    • Anti-SYVN1/HRD1 antibody [EP7459] - BSA and Azide free (ab249578)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • Ramos whole cell lysate (ab3955)
  • Recombinant Protein

    • Recombinant Human SYVN1/HRD1 protein (ab132449)
  • Related Products

    • Recombinant Human SYVN1/HRD1 protein (ab132449)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the HRD1 family.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger is required for E3 ligase activity.
  • Post-translational
    modifications

    Not N-glycosylated.
    Auto-ubiquitinated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession Q86TM6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 84447 Human
    • Entrez Gene: 74126 Mouse
    • Entrez Gene: 361712 Rat
    • Omim: 608046 Human
    • SwissProt: Q86TM6 Human
    • SwissProt: Q9DBY1 Mouse
    • Unigene: 75859 Human
    • Unigene: 149870 Mouse
    • Alternative names

      • 1200010C09Rik antibody
      • DER3 antibody
      • E3 ubiquitin-protein ligase synoviolin antibody
      • HMG coA reductase degradation 1 homolog antibody
      • HRD1 antibody
      • KIAA1810 antibody
      • MGC40372 antibody
      • OTTHUMP00000230429 antibody
      • OTTHUMP00000230430 antibody
      • OTTHUMP00000230431 antibody
      • OTTHUMP00000230432 antibody
      • Synovial apoptosis inhibitor 1 antibody
      • Synovial apoptosis inhibitor 1, synoviolin antibody
      • Synoviolin 1 antibody
      • Synoviolin 1 isoform b antibody
      • SYNOVIOLIN antibody
      • SYVN1 antibody
      • SYVN1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
      Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
      All lanes : Anti-SYVN1/HRD1 antibody [EP7459] (ab170901) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : HepG2 cell line lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labeled goat anti-rabbit. at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 67 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
      Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)

      Immunofluorescence analysis of HeLa cells labeling SYVN1/HRD1, using ab170901 at a 1/100 dilution (red), and DAPI nuclear staining (blue).

    • Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
      Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)

      Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling SYVN1/HRD1 using ab170901 at a 1/10 dilution

    • Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
      Flow Cytometry (Intracellular) - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)

      Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling SYVN1/HRD1 (red), using ab170901 at a 1/10 dilution, or a negative control rabbit IgG (green). 

       

       

    • Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
      Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (10)

    Publishing research using ab170901? Please let us know so that we can cite the reference in this datasheet.

    ab170901 has been referenced in 10 publications.

    • Liu S  et al. TRIM27 acts as an oncogene and regulates cell proliferation and metastasis in non-small cell lung cancer through SIX3-ß-catenin signaling. Aging (Albany NY) 12:25564-25580 (2020). PubMed: 33264103
    • He YX  et al. Zonisamide Ameliorates Cognitive Impairment by Inhibiting ER Stress in a Mouse Model of Type 2 Diabetes Mellitus. Front Aging Neurosci 12:192 (2020). PubMed: 32754028
    • Jin Y  et al. The role of Hrd1 in ultraviolet (UV) radiation induced photoaging. Aging (Albany NY) 12:21273-21289 (2020). PubMed: 33168784
    • Xu BY  et al. Rifampicin induces clathrin-dependent endocytosis and ubiquitin-proteasome degradation of MRP2 via oxidative stress-activated PKC-ERK/JNK/p38 and PI3K signaling pathways in HepG2 cells. Acta Pharmacol Sin N/A:N/A (2019). PubMed: 31316180
    • Cheng X  et al. ATRA protects skin fibroblasts against UV-induced oxidative damage through inhibition of E3 ligase Hrd1. Mol Med Rep 20:2294-2302 (2019). PubMed: 31322186
    • Bushell SR  et al. The structural basis of lipid scrambling and inactivation in the endoplasmic reticulum scramblase TMEM16K. Nat Commun 10:3956 (2019). PubMed: 31477691
    • Xu ZM  et al. Ginsenoside Rg1 Prevents Doxorubicin-Induced Cardiotoxicity through the Inhibition of Autophagy and Endoplasmic Reticulum Stress in Mice. Int J Mol Sci 19:N/A (2018). PubMed: 30463294
    • Feng L  et al. Ubiquitin ligase SYVN1/HRD1 facilitates degradation of the SERPINA1 Z variant/a-1-antitrypsin Z variant via SQSTM1/p62-dependent selective autophagy. Autophagy 13:686-702 (2017). PubMed: 28121484
    • Kong S  et al. Endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 controls B-cell immunity through degradation of the death receptor CD95/Fas. Proc Natl Acad Sci U S A 113:10394-9 (2016). PubMed: 27573825
    • Shen H  et al. Endoplasmic Reticulum Stress Induces the Early Appearance of Pro-apoptotic and Anti-apoptotic Proteins in Neurons of Five Familial Alzheimer's Disease Mice. Chin Med J (Engl) 129:2845-2852 (2016). PubMed: 27901000

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