Recombinant Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-SYVN1/HRD1 antibody [EP7459]
See all SYVN1/HRD1 primary antibodies -
Description
Rabbit monoclonal [EP7459] to SYVN1/HRD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SYVN1/HRD1 aa 150-250 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
Database link: Q86TM6 -
Positive control
- 293T, HepG2, HeLa, Ramos and SH-SY5Y cell lysates, HeLa and 293T cells.
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General notes
This product was previously labelled as SYVN1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP7459 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab170901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 67 kDa. | |
| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IP | 1/10 - 1/100. |
Target
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Function
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. -
Tissue specificity
Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level). -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the HRD1 family.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger is required for E3 ligase activity. -
Post-translational
modificationsNot N-glycosylated.
Auto-ubiquitinated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 84447 Human
- Entrez Gene: 74126 Mouse
- Entrez Gene: 361712 Rat
- Omim: 608046 Human
- SwissProt: Q86TM6 Human
- SwissProt: Q9DBY1 Mouse
- Unigene: 75859 Human
- Unigene: 149870 Mouse
see all -
Alternative names
- 1200010C09Rik antibody
- DER3 antibody
- E3 ubiquitin-protein ligase synoviolin antibody
see all
Images
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![Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.com/ps/products/170/ab170901/Images/ab170901-172098-anti-syvn1-antibody-ep7459-western-blot.jpg)
Western blot - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)All lanes : Anti-SYVN1/HRD1 antibody [EP7459] (ab170901) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HepG2 cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit. at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.com/ps/products/170/ab170901/Images/ab170901-172100-anti-syvn1-antibody-ep7459-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)Immunofluorescence analysis of HeLa cells labeling SYVN1/HRD1, using ab170901 at a 1/100 dilution (red), and DAPI nuclear staining (blue).
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![Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.com/ps/products/170/ab170901/Images/ab170901-172099-anti-syvn1-antibody-ep7459-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling SYVN1/HRD1 using ab170901 at a 1/10 dilution
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![Flow Cytometry - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)](https://www.abcam.com/ps/products/170/ab170901/Images/ab170901-172097-anti-syvn1-antibody-ep7459-flow-cytometry.jpg)
Flow Cytometry - Anti-SYVN1/HRD1 antibody [EP7459] (ab170901)Flow cytometry analysis of permeabilized 293T cells labeling SYVN1/HRD1 (red), using ab170901 at a 1/10 dilution, or a negative control rabbit IgG (green).
Protocols
References
This product has been referenced in:
- Xu ZM et al. Ginsenoside Rg1 Prevents Doxorubicin-Induced Cardiotoxicity through the Inhibition of Autophagy and Endoplasmic Reticulum Stress in Mice. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 30463294) »
- Feng L et al. Ubiquitin ligase SYVN1/HRD1 facilitates degradation of the SERPINA1 Z variant/a-1-antitrypsin Z variant via SQSTM1/p62-dependent selective autophagy. Autophagy 13:686-702 (2017). Read more (PubMed: 28121484) »
