Product nameT-47D nuclear extract lysate
See all T47D lysates
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
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BackgroundT47D cells carry receptors for a variety of steroids and calcitonin. They express mutant tumor suppressor protein p53 protein. Under normal culturing conditions, they express progesterone receptor constitutively and are responsive to estrogen. They are able to lose the estrogen receptor (ER) during long-term estrogen deprivation in vitro. Cells are therefore used as a model for studies of drug resistance to tamoxifen in patients with mutant p53 breast tumors.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab14896 has been referenced in 1 publication.
- Lockwood CJ et al. Human labor is associated with reduced decidual cell expression of progesterone, but not glucocorticoid, receptors. J Clin Endocrinol Metab 95:2271-5 (2010). WB . PubMed: 20237167