Key features and details
- Mouse monoclonal [4B10] to T-bet / Tbx21
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-T-bet / Tbx21 antibody [4B10]
See all T-bet / Tbx21 primary antibodies
DescriptionMouse monoclonal [4B10] to T-bet / Tbx21
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
E. coli protein
- Th1 polarized Mouse CD4+ T cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab91109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 58 kDa.|
|Flow Cyt||Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionTranscription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
Tissue specificityT-cell specific.
Involvement in diseaseGenetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.
Sequence similaritiesContains 1 T-box DNA-binding domain.
- Information by UniProt
- T bet antibody
- T box 21 antibody
- T box expressed in T cells antibody
IHC image of T-bet / Tbx21 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91109, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-T-bet / Tbx21 antibody [4B10] (ab91109) at 2 µg/ml
Lane 1 : Lysates of CD4+ T cells (control)
Lane 2 : Lysates of PMA and Ionomycin reactivated CD4+ T cells
All lanes : HRP anti-mouse IgG
Predicted band size: 58 kDa
CD4+ T cells were sorted from Mouse spleen, activated with anti-mouse CD3 and anti-mouse CD28, followed by culture in Th1 polarizing conditions, and reactivation with PMA and Ionomycin.
ab91109 staining T-bet in Human acne lesion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formaldehyde and antigen retrieval was by heat mediation perfomred in a microwave oven. Samples were incubated with primary antibody (1/200).
Overlay histogram showing Jurkat cells stained with ab91109 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91109, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab91109 has been referenced in 15 publications.
- Yu Q et al. Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain. Brain Struct Funct N/A:N/A (2019). PubMed: 30604007
- Nicastro M et al. Fibrocytes in Chronic Periaortitis: A Novel Mechanism Linking Inflammation and Fibrosis. Arthritis Rheumatol 71:1913-1922 (2019). PubMed: 31233292
- Zhu Q et al. Andrographolide affects Th1/Th2/Th17 responses of peripheral blood mononuclear cells from ulcerative colitis patients. Mol Med Rep 18:622-626 (2018). PubMed: 29749556
- Naing A et al. PEGylated IL-10 (Pegilodecakin) Induces Systemic Immune Activation, CD8+ T Cell Invigoration and Polyclonal T Cell Expansion in Cancer Patients. Cancer Cell 34:775-791.e3 (2018). PubMed: 30423297
- De-Pu Z et al. The cholinergic anti-inflammatory pathway ameliorates acute viral myocarditis in mice by regulating CD4+ T cell differentiation. Virulence 9:1364-1376 (2018). PubMed: 30176160