Recombinant
RabMAb

Recombinant Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)

Overview

  • Product name

    Anti-T-bet / Tbx21 antibody [EPR9301]
    See all T-bet / Tbx21 primary antibodies
  • Description

    Rabbit monoclonal [EPR9301] to T-bet / Tbx21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human T-bet/ Tbx21 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9UL17

  • Positive control

    • Human T cell lymphoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250-1/500.

See IHC antigen retrieval protocols.

Target

  • Function

    Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
  • Tissue specificity

    T-cell specific.
  • Involvement in disease

    Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.
  • Sequence similarities

    Contains 1 T-box DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • T bet antibody
    • T box 21 antibody
    • T box expressed in T cells antibody
    • T box protein 21 antibody
    • T box transcription factor TBX21 antibody
    • T cell specific T box transcription factor antibody
    • T cell specific T box transcription factor T bet antibody
    • T PET antibody
    • T-box protein 21 antibody
    • T-box transcription factor TBX21 antibody
    • T-cell-specific T-box transcription factor T-bet antibody
    • TBET antibody
    • TBLYM antibody
    • TBX 21 antibody
    • Tbx21 antibody
    • TBX21_HUMAN antibody
    • TPET antibody
    • Transcription factor TBLYM antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human T cell lymphoma tissue labelling T-bet / Tbx21 with unpurified ab150440 at 1/250 dilution.

  • Immunohistochemical analysis of paraffin embedded Human Hodgkin's lymphoma tissue using unpurified ab150440 showing +ve staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Hodgkin lymphoma tissue sections labeling T-bet/Tbx21 with Purified ab150440 at 1:1000 dilution (1.23 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemical analysis of paraffin embedded Human Normal spleen tissue using unpurified ab150440 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using unpurified ab150440 showing -ve staining.

  • Immunohistochemical analysis of paraffin embedded Human T cell lymphoma tissue using unpurified ab150440 showing +ve staining.

References

This product has been referenced in:

  • Wang C  et al. Immune profiling of microsatellite instability-high and polymerase e (POLE)-mutated metastatic colorectal tumors identifies predictors of response to anti-PD-1 therapy. J Gastrointest Oncol 9:404-415 (2018). IHC-P ; Human . Read more (PubMed: 29998005) »
  • Child KM  et al. The Neuroregenerative Capacity of Olfactory Stem Cells Is Not Limitless: Implications for Aging. J Neurosci 38:6806-6824 (2018). Read more (PubMed: 29934351) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymphoid tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 EDTA Leica buffer
Permeabilization
No
Specification
Lymphoid tissue
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 28 2016

Answer

We did test the antibody for western blotting, but it tested negative. The blot was blank on Jurkat, HuT-78 and Molt-4 lysates.

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