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    t-bet--tbx21-antibody-epr9301-ab150440.pdf

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Immunology Adaptive Immunity B Cells Non-CD
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RecombinantRabMAb

Recombinant Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
  • Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9301] to T-bet / Tbx21
  • Suitable for: IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-T-bet / Tbx21 antibody [EPR9301]
    See all T-bet / Tbx21 primary antibodies
  • Description

    Rabbit monoclonal [EPR9301] to T-bet / Tbx21
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human T-bet/ Tbx21 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9UL17

  • Positive control

    • IHC-P: Human T cell lymphoma, Hodgkin's lymphoma and spleen tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9301
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-T-bet / Tbx21 antibody [EPR9301] (ab225198)
    • Alexa Fluor® 488 Anti-T-bet / Tbx21 antibody [EPR9301] (ab225199)
    • Anti-T-bet / Tbx21 antibody [EPR9301] - BSA and Azide free (ab227115)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human T-bet / Tbx21 protein (ab182815)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
Application Abreviews Notes
IHC-P (1)
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250-1/500.

See IHC antigen retrieval protocols.

Notes
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250-1/500.

See IHC antigen retrieval protocols.

Target

  • Function

    Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
  • Tissue specificity

    T-cell specific.
  • Involvement in disease

    Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.
  • Sequence similarities

    Contains 1 T-box DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9UL17 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 30009 Human
    • Omim: 604895 Human
    • SwissProt: Q9UL17 Human
    • Unigene: 272409 Human
    • Alternative names

      • T bet antibody
      • T box 21 antibody
      • T box expressed in T cells antibody
      • T box protein 21 antibody
      • T box transcription factor TBX21 antibody
      • T cell specific T box transcription factor antibody
      • T cell specific T box transcription factor T bet antibody
      • T PET antibody
      • T-box protein 21 antibody
      • T-box transcription factor TBX21 antibody
      • T-cell-specific T-box transcription factor T-bet antibody
      • TBET antibody
      • TBLYM antibody
      • TBX 21 antibody
      • Tbx21 antibody
      • TBX21_HUMAN antibody
      • TPET antibody
      • Transcription factor TBLYM antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human T cell lymphoma tissue labelling T-bet / Tbx21 with unpurified ab150440 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)

      Immunohistochemical analysis of paraffin embedded Human Hodgkin's lymphoma tissue using unpurified ab150440 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Hodgkin lymphoma tissue sections labeling T-bet/Tbx21 with Purified ab150440 at 1:1000 dilution (1.23 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)

      Immunohistochemical analysis of paraffin embedded Human Normal spleen tissue using unpurified ab150440 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)

      Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using unpurified ab150440 showing -ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)

      Immunohistochemical analysis of paraffin embedded Human T cell lymphoma tissue using unpurified ab150440 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)
      Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab150440? Please let us know so that we can cite the reference in this datasheet.

    ab150440 has been referenced in 5 publications.

    • Wang C  et al. Immune profiling of microsatellite instability-high and polymerase e (POLE)-mutated metastatic colorectal tumors identifies predictors of response to anti-PD-1 therapy. J Gastrointest Oncol 9:404-415 (2018). IHC-P ; Human . PubMed: 29998005
    • Child KM  et al. The Neuroregenerative Capacity of Olfactory Stem Cells Is Not Limitless: Implications for Aging. J Neurosci 38:6806-6824 (2018). PubMed: 29934351
    • Komura T  et al. Clinical features of cystatin A expression in patients with pancreatic ductal adenocarcinoma. Cancer Sci 108:2122-2129 (2017). IHC ; Human . PubMed: 28898495
    • Zhang W  et al. GATA3 expression correlates with poor prognosis and tumor-associated macrophage infiltration in peripheral T cell lymphoma. Oncotarget 7:65284-65294 (2016). PubMed: 27589565
    • Lau MC  et al. Genetic association of ankylosing spondylitis with TBX21 influences T-bet and pro-inflammatory cytokine expression in humans and SKG mice as a model of spondyloarthritis. Ann Rheum Dis N/A:N/A (2016). IHC ; Human . PubMed: 27125523

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-T-bet / Tbx21 antibody [EPR9301]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lymphoid tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH6 EDTA Leica buffer
    Permeabilization
    No
    Specification
    Lymphoid tissue
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 28 2016

    Question

    Did you check Anti-T-bet / Tbx21 antibody (ab150440) for WB?
    I just find only histology picture on abcam website.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 01 2013

    Answer

    We did test the antibody for western blotting, but it tested negative. The blot was blank on Jurkat, HuT-78 and Molt-4 lysates.

    Read More

    Abcam Scientific Support

    Answered on Mar 01 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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