Recombinant
RabMAb

Recombinant Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)

Overview

  • Product name

    Anti-T-bet / Tbx21 antibody [EPR9302]
    See all T-bet / Tbx21 primary antibodies
  • Description

    Rabbit monoclonal [EPR9302] to T-bet / Tbx21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: ICC,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human T-bet/ Tbx21. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Hodgkin's Lymphoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC,IP or WB.
  • Target

    • Function

      Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
    • Tissue specificity

      T-cell specific.
    • Involvement in disease

      Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.
    • Sequence similarities

      Contains 1 T-box DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • T bet antibody
      • T box 21 antibody
      • T box expressed in T cells antibody
      • T box protein 21 antibody
      • T box transcription factor TBX21 antibody
      • T cell specific T box transcription factor antibody
      • T cell specific T box transcription factor T bet antibody
      • T PET antibody
      • T-box protein 21 antibody
      • T-box transcription factor TBX21 antibody
      • T-cell-specific T-box transcription factor T-bet antibody
      • TBET antibody
      • TBLYM antibody
      • TBX 21 antibody
      • Tbx21 antibody
      • TBX21_HUMAN antibody
      • TPET antibody
      • Transcription factor TBLYM antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin lymphoma tissue sections labeling T-bet / Tbx21 with Purified ab154200 at 1:500 dilution (0.68 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma labeling T-bet/Tbx21 with ab154200 at 1/250 dilution

    References

    ab154200 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate , pressure cooker
    Permeabilization
    No
    Specification
    pancreas
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    zinc

    Dr. Thea Golden

    Verified customer

    Submitted Feb 22 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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