Recombinant Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)](https://www.abcam.com/ps/products/154/ab154200/Images/ab154200-318896-anti-t-bet-tbx21-antibody-epr9302-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9302] to T-bet / Tbx21
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-T-bet / Tbx21 antibody [EPR9302]
See all T-bet / Tbx21 primary antibodies -
Description
Rabbit monoclonal [EPR9302] to T-bet / Tbx21 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: ICC,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human T-bet/ Tbx21. The exact sequence is proprietary.
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Positive control
- IHC-P: Hodgkin's Lymphoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9302 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (4) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. -
Tissue specificity
T-cell specific. -
Involvement in disease
Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities. -
Sequence similarities
Contains 1 T-box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 30009 Human
- Omim: 604895 Human
- SwissProt: Q9UL17 Human
- Unigene: 272409 Human
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Alternative names
- T bet antibody
- T box 21 antibody
- T box expressed in T cells antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin lymphoma tissue sections labeling T-bet / Tbx21 with Purified ab154200 at 1:500 dilution (0.68 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)](https://www.abcam.com/ps/products/154/ab154200/Images/ab154200-160189-anti-t-bet-tbx21-antibody-epr9302-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma labeling T-bet/Tbx21 with ab154200 at 1/250 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)](https://www.abcam.com/ps/products/154/ab154200/Images/ab154200-4-benefits-of-recombinant-antibodies.png)
Anti-T-bet / Tbx21 antibody [EPR9302] (ab154200)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154200 has not yet been referenced specifically in any publications.