Key features and details
- Mouse monoclonal [39D, 3-9D] to T-bet / Tbx21
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-T-bet / Tbx21 antibody [39D, 3-9D]
See all T-bet / Tbx21 primary antibodies
DescriptionMouse monoclonal [39D, 3-9D] to T-bet / Tbx21
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant protein purified from E.coli
- Th1-polarized mouse CD4+ T cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Clone number39D, 3-9D
Our Abpromise guarantee covers the use of ab91103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 58 kDa.|
FunctionTranscription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
Tissue specificityT-cell specific.
Involvement in diseaseGenetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.
Sequence similaritiesContains 1 T-box DNA-binding domain.
- Information by UniProt
- T bet antibody
- T box 21 antibody
- T box expressed in T cells antibody
All lanes : Anti-T-bet / Tbx21 antibody [39D, 3-9D] (ab91103) at 2 µg/ml
Lane 1 : Lysates from control cells
Lane 2 : Lysates from PMA and Ionomycin-re-activated cells
All lanes : HRP anti-mouse IgG
Predicted band size: 58 kDa
CD4+ T cells were sorted from mouse spleen, activated with anti-mouse CD3 and anti-mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation with PMA and Ionomycin.
ab91103 has been referenced in 2 publications.
- Spurlock CF et al. Profiles of Long Noncoding RNAs in Human Naive and Memory T Cells. J Immunol 199:547-558 (2017). PubMed: 28600289
- Shao YY et al. The Anti-Allergic Rhinitis Effect of Traditional Chinese Medicine of Shenqi by Regulating Mast Cell Degranulation and Th1/Th2 Cytokine Balance. Molecules 22:N/A (2017). PubMed: 28327534