Anti-T-bet / Tbx21 antibody [4B10] (ab91109)

Mouse monoclonal T-bet / Tbx21 antibody [4B10]. Validated in WB, IP, IHC, Flow Cyt and tested in Mouse, Human. Cited in 9 publication(s). Independently reviewed in 2 review(s).

Overview

  • Product name

    Anti-T-bet / Tbx21 antibody [4B10]
    See all T-bet / Tbx21 primary antibodies
  • Description

    Mouse monoclonal [4B10] to T-bet / Tbx21
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    E. coli protein

  • Positive control

    • Th1 polarized Mouse CD4+ T cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab91109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 58 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
  • Tissue specificity

    T-cell specific.
  • Involvement in disease

    Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.
  • Sequence similarities

    Contains 1 T-box DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • T bet antibody
    • T box 21 antibody
    • T box expressed in T cells antibody
    • T box protein 21 antibody
    • T box transcription factor TBX21 antibody
    • T cell specific T box transcription factor antibody
    • T cell specific T box transcription factor T bet antibody
    • T PET antibody
    • T-box protein 21 antibody
    • T-box transcription factor TBX21 antibody
    • T-cell-specific T-box transcription factor T-bet antibody
    • TBET antibody
    • TBLYM antibody
    • TBX 21 antibody
    • Tbx21 antibody
    • TBX21_HUMAN antibody
    • TPET antibody
    • Transcription factor TBLYM antibody
    see all

Images

  • All lanes : Anti-T-bet / Tbx21 antibody [4B10] (ab91109) at 2 µg/ml

    Lane 1 : Lysates of CD4+ T cells (control)
    Lane 2 : Lysates of PMA and Ionomycin reactivated CD4+ T cells

    Secondary
    All lanes : HRP anti-mouse IgG

    Predicted band size: 58 kDa



    CD4+ T cells were sorted from Mouse spleen, activated with anti-mouse CD3 and anti-mouse CD28, followed by culture in Th1 polarizing conditions, and reactivation with PMA and Ionomycin.
  • IHC image of T-bet / Tbx21 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91109, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab91109 staining T-bet in Human acne lesion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formaldehyde and antigen retrieval was by heat mediation perfomred in a microwave oven. Samples were incubated with primary antibody (1/200).

  • Overlay histogram showing Jurkat cells stained with ab91109 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91109, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:

  • Yu Q  et al. Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain. Brain Struct Funct N/A:N/A (2019). Read more (PubMed: 30604007) »
  • De-Pu Z  et al. The cholinergic anti-inflammatory pathway ameliorates acute viral myocarditis in mice by regulating CD4+ T cell differentiation. Virulence 9:1364-1376 (2018). Read more (PubMed: 30176160) »
See all 11 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (CD3 T cells isolated from mouse gut)
Specification
CD3 T cells isolated from mouse gut
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 15 2014

Question
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab91109 with the order number xxxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (human gut biopsies)
Loading amount
100 µg
Specification
human gut biopsies
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Oct 30 2010

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