Key features and details
- Rabbit polyclonal to T-bet / Tbx21 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-T-bet / Tbx21 antibody - C-terminal
See all T-bet / Tbx21 primary antibodies
DescriptionRabbit polyclonal to T-bet / Tbx21 - C-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human T-bet/ Tbx21 (C terminal). (19 amino acid peptide; NP_037483).
Database link: Q9UL17
- 293 cells and cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab181400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionTranscription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
Tissue specificityT-cell specific.
Involvement in diseaseGenetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.
Sequence similaritiesContains 1 T-box DNA-binding domain.
- Information by UniProt
- T bet antibody
- T box 21 antibody
- T box expressed in T cells antibody
Anti-T-bet / Tbx21 antibody - C-terminal (ab181400) at 1 µg/ml + 293 cell lysate at 15 µg
Predicted band size: 58 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Immunofluorescent analysis of 293 cells labeling T-bet / Tbx21 with ab181400 at 5 µg/ml.
ab181400 has not yet been referenced specifically in any publications.