Product nameAnti-T Plastin antibody
See all T Plastin primary antibodies
DescriptionRabbit polyclonal to T Plastin
SpecificityThis antibody is specific for T Plastin.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to the N-terminal of human T Plastin.
- T plastin expressing bacterial extract, Sezary cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab45769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 70 kDa.|
|ELISA||1/2000 - 1/5000.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.
Tissue specificityExpressed in a variety of organs, including muscle, brain, uterus and esophagus.
Sequence similaritiesContains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- BMND18 antibody
- Plastin 3 (T isoform) antibody
- Plastin 3 antibody
All lanes : Anti-T Plastin antibody (ab45769) at 1/1000 dilution
Lane 1 : Bacterial extract expressing T-Plastin at 1 µg
Lane 2 : Bacterial extract expressing L-Plastin at 1 µg
Lane 3 : Bacterial extract expressing I-Plastin at 1 µg
Lane 4 : Sezary cell extract at 10 µg
Lane 5 : Control T-Helper cells at 10 µg
Predicted band size: 70 kDa
Observed band size: 70 kDa
IHC image of ab45769 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45769, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Jones CL et al. Regulation of T-Plastin Expression by Promoter Hypomethylation in Primary Cutaneous T-Cell Lymphoma. J Invest Dermatol : (2012). Human . Read more (PubMed: 22495182) »
- Bollinger KE et al. Proteomic similarities in steroid responsiveness in normal and glaucomatous trabecular meshwork cells. Mol Vis 18:2001-11 (2012). WB ; Human . Read more (PubMed: 22876128) »