• Product name
  • Description
    Rabbit polyclonal to T Plastin
  • Host species
  • Specificity
    This antibody is specific for T Plastin.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to the N-terminal of human T Plastin.

  • Positive control
    • T plastin expressing bacterial extract, Sezary cells



Our Abpromise guarantee covers the use of ab45769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 70 kDa.
ELISA 1/2000 - 1/5000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.
  • Tissue specificity
    Expressed in a variety of organs, including muscle, brain, uterus and esophagus.
  • Sequence similarities
    Contains 2 actin-binding domains.
    Contains 4 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BMND18 antibody
    • Plastin 3 (T isoform) antibody
    • Plastin 3 antibody
    • Plastin-3 antibody
    • PLS3 antibody
    • PLST_HUMAN antibody
    • T fimbrin antibody
    • T-plastin antibody
    see all


  • All lanes : Anti-T Plastin antibody (ab45769) at 1/1000 dilution

    Lane 1 : Bacterial extract expressing T-Plastin at 1 µg
    Lane 2 : Bacterial extract expressing L-Plastin at 1 µg
    Lane 3 : Bacterial extract expressing I-Plastin at 1 µg
    Lane 4 : Sezary cell extract at 10 µg
    Lane 5 : Control T-Helper cells at 10 µg

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

  • IHC image of ab45769 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45769, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Jones CL  et al. Regulation of T-Plastin Expression by Promoter Hypomethylation in Primary Cutaneous T-Cell Lymphoma. J Invest Dermatol : (2012). Human . Read more (PubMed: 22495182) »
  • Bollinger KE  et al. Proteomic similarities in steroid responsiveness in normal and glaucomatous trabecular meshwork cells. Mol Vis 18:2001-11 (2012). WB ; Human . Read more (PubMed: 22876128) »

See all 2 Publications for this product

Customer reviews and Q&As

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The lead time for this product is 1-2 weeks.

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The antibody should detect a single band however the multiple bands proble...

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I am happy to provide the free of charge replacement. However we do have another antibody ab128690 which has quite a promising WB image, would you like to try this one.

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