Key features and details
- Rabbit polyclonal to T Plastin/PLS3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-T Plastin/PLS3 antibody
See all T Plastin/PLS3 primary antibodies
DescriptionRabbit polyclonal to T Plastin/PLS3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, Pig
Predicted to work with: Rat, Cow, Chinese hamster
Recombinant fragment (His-tag) corresponding to Human T Plastin/PLS3 aa 379-630. (Expressed in E.coli).
ALTKPENQDIDWTLLEGETREERTFRNWMNSLGVNPHVNHLYADLQDALV ILQLYERIKVPVDWSKVNKPPYPKLGANMKKLENCNYAVELGKHPAKFSL VGIGGQDLNDGNQTLTLALVWQLMRRYTLNVLEDLGDGQKANDDIIVNWV NRTLSEAGKSTSIQSFKDKTISSSLAVVDLIDAIQPGCINYDLVKSGNLT EDDKHNNAKYAVSMARRIGARVYALPEDLVEVKPKMVMTVFACLMGRGMK RV
Database link: P13797
- WB: Pig liver and heart lysate; Mouse liver lysate; Recombinant human T Plastin/PLS3 protein. IHC-P: Human prostate cancer, stomach cancer and rectum cancer tissue.
This product was previously labelled as T Plastin
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab233104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 71 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionActin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.
Tissue specificityExpressed in a variety of organs, including muscle, brain, uterus and esophagus.
Sequence similaritiesContains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- BMND18 antibody
- Plastin 3 (T isoform) antibody
- Plastin 3 antibody
Formalin-fixed, paraffin-embedded human rectum cancer tissue stained for T Plastin/PLS3 using ab233104 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for T Plastin/PLS3 using ab233104 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for T Plastin/PLS3 using ab233104 at 10 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-T Plastin/PLS3 antibody (ab233104) at 2 µg/ml
Lane 1 : Pig liver tissue
Lane 2 : Pig heart tissue
Lane 3 : Mouse liver tissue
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 71 kDa
Anti-T Plastin/PLS3 antibody (ab233104) at 2 µg/ml + Recombinant human T Plastin/PLS3 protein
Predicted band size: 71 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233104 has not yet been referenced specifically in any publications.