Anti-T1R3 antibody (ab150525)
Key features and details
- Rabbit polyclonal to T1R3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-T1R3 antibody
See all T1R3 primary antibodies -
Description
Rabbit polyclonal to T1R3 -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Baboon, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human T1R3 (N terminal).
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Positive control
- Human tongue, squamous epithelium.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose (By similarity). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 100610821 Chimpanzee
- Entrez Gene: 83756 Human
- Omim: 605865 Human
- SwissProt: Q717C2 Chimpanzee
- SwissProt: Q717C1 Gorilla
- SwissProt: Q7RTX0 Human
- Unigene: 74375 Human
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Alternative names
- G protein coupled receptor antibody
- Sac antibody
- Saccharin preference antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tongue, squamous epithelium labelling T1R3 with ab150525 at 17 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling T1R3 with ab150525. Heat-induced antigen retrieval was performed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab150525 has been referenced in 4 publications.
- Gong T et al. Longitudinal Expression of Testicular TAS1R3 from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs. Animals (Basel) 11:N/A (2021). PubMed: 33567555
- Shen T & Li J Drinking Non-nutritive Sweetness Solution of Sodium Saccharin or Rebaudioside a for Guinea Pigs: Influence on Histologic Change and Expression of Sweet Taste Receptors in Testis and Epididymis. Front Nutr 8:720889 (2021). PubMed: 34422887
- Tian M et al. Dietary Branched-Chain Amino Acids Regulate Food Intake Partly through Intestinal and Hypothalamic Amino Acid Receptors in Piglets. J Agric Food Chem 67:6809-6818 (2019). PubMed: 31134808
- Tian M et al. Branched chain amino acids stimulate gut satiety hormone cholecystokinin secretion through activation of the umami taste receptor T1R1/T1R3 using an in vitro porcine jejunum model. Food Funct 10:3356-3367 (2019). PubMed: 31098606