Key features and details
- Rabbit polyclonal to T1R3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-T1R3 antibody
See all T1R3 primary antibodies
DescriptionRabbit polyclonal to T1R3
SpecificityBLAST analysis of the peptide immunogen showed no homology with other Human proteins.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Baboon, Gorilla, Orangutan
Synthetic peptide corresponding to Human T1R3 (N terminal).
- Human tongue, squamous epithelium.
Protein previously labeled as GPCR TAS1R3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionPutative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose (By similarity). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family. TAS1R subfamily.
Cellular localizationCell membrane.
- Information by UniProt
- G protein coupled receptor antibody
- Sac antibody
- Saccharin preference antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tongue, squamous epithelium labelling T1R3 with ab150525 at 17 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling T1R3 with ab150525. Heat-induced antigen retrieval was performed.
ab150525 has been referenced in 2 publications.
- Tian M et al. Dietary Branched-Chain Amino Acids Regulate Food Intake Partly through Intestinal and Hypothalamic Amino Acid Receptors in Piglets. J Agric Food Chem 67:6809-6818 (2019). PubMed: 31134808
- Tian M et al. Branched chain amino acids stimulate gut satiety hormone cholecystokinin secretion through activation of the umami taste receptor T1R1/T1R3 using an in vitro porcine jejunum model. Food Funct 10:3356-3367 (2019). PubMed: 31098606