Overview

  • Product name

  • Description

    Rabbit polyclonal to T1R3
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Baboon, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human T1R3 (N terminal).

  • Positive control

    • Human tongue, squamous epithelium.
  • General notes

    Protein previously labeled as GPCR TAS1R3.

Properties

Applications

Our Abpromise guarantee covers the use of ab150525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose (By similarity). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • G protein coupled receptor antibody
    • Sac antibody
    • Saccharin preference antibody
    • Sweet taste receptor T1R3 antibody
    • T1 R3 antibody
    • T1R 3 antibody
    • T1R3 antibody
    • TAS1 R3 antibody
    • TAS1R 3 antibody
    • TAS1R3 antibody
    • Taste receptor type 1 member 3 antibody
    • Taste receptor type 1 member 3 precursor antibody
    • TR 3 antibody
    • TR3 antibody
    • TS1R3_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tongue, squamous epithelium labelling T1R3 with ab150525 at 17 µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling T1R3 with ab150525. Heat-induced antigen retrieval was performed.

References

ab150525 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Human Cell (Primary lung cancer cell lines (LT215) human lung)
Permeabilization
No
Gating Strategy
gate on morphological parameters and 7-ADD negative cells
Specification
Primary lung cancer cell lines (LT215) human lung
Preparation
Cell harvesting/tissue preparation method: Cancer cells were cultured in adhesion in RPMI+10% fetal bovin serum. Cells were harvested by trypsinization and washed in staining buffer.
Sample buffer: staining buffer: PBS1X+0,5%BSA+2mEDTA
Fixation
No fixation

Mrs. María de la Fuente

Verified customer

Submitted May 23 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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