Overview

  • Product name
    T47D nuclear extract lysate
    See all T47D lysates
  • General notes
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • Background
    T47D cells carry receptors for a variety of steroids and calcitonin. They express mutant tumor suppressor protein p53 protein. Under normal culturing conditions, they express progesterone receptor constitutively and are responsive to estrogen. They are able to lose the estrogen receptor (ER) during long-term estrogen deprivation in vitro. Cells are therefore used as a model for studies of drug resistance to tamoxifen in patients with mutant p53 breast tumors.

References

This product has been referenced in:
  • Lockwood CJ  et al. Human labor is associated with reduced decidual cell expression of progesterone, but not glucocorticoid, receptors. J Clin Endocrinol Metab 95:2271-5 (2010). WB . Read more (PubMed: 20237167) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Regarding ab14899, the procedure for the preparation of the whole cell extract is not RNAase free so the RNA will be at least partially degraded. But there is no step to get rid of the RNA and since it’s more soluble than DNA it could be present in the extract. We have never tested our extract for the presence of RNA and therefore we can not guarantee that it will be RNA free.

Unfortunately, we haven’t tested the presence of organelles either.

We have two nuclear lysates: ab14896 and ab14897 (IFN gamma treated).

These nuclear extracts don't contain any cytoplasmic proteins, the nuclei are first isolated then lysed. IFN gamma will affect the expression of some proteins. For example, they activate the expression of inflammation-related proteins.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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