Key features and details
- Rabbit polyclonal to T54
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-T54 antibody
See all T54 primary antibodies
DescriptionRabbit polyclonal to T54
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: A549 cell lysate. ICC/IF: U-2 OS cells. IHC-P: Human fallopian tube tissue.
Previously labelled as GPKOW.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 52 kDa.|
Sequence similaritiesBelongs to the MOS2 family.
Contains 1 G-patch domain.
Contains 2 KOW domains.
- Information by UniProt
- G patch domain and KOW motifs antibody
- G patch domain and KOW motifs containing protein antibody
- G patch domain and KOW motifs-containing protein antibody
Anti-T54 antibody (ab224315) at 1/250 dilution + A549 (human lung carcinoma cell line) cell lysate
Predicted band size: 52 kDa
Paraffin embedded human fallopian tube tissue stained for T54 with ab224315 (1/50 dilution) in immunohistochemical analysis.
PFA-fixed/Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for T54 (green) using ab224315 (4 µg/ml) in ICC/IF.
ab224315 has not yet been referenced specifically in any publications.