Overview

  • Product name

  • Description

    Goat polyclonal to T7 tag®
  • Host species

    Goat
  • Specificity

    This antibody was verified by ELISA against peptide conjugated to BSA(MASMTGGQQMG/BSA). Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • Tested applications

    Suitable for: ELISA, IP, WB, ICC, ChIPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide:

    MASMTGGQQMG

    (T7) conjugated to KLH.

  • General notes

    T7-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was affinity purified using the peptide immobilized on solid support..
  • Clonality

    Polyclonal
  • Isotype

    unknown
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Use at a dilution of: Coating 1/100 - 1/500, Primary 1/1000 - 1/30,000.
IP Use at an assay dependent dilution.
WB 1/1000 - 1/30000.
ICC 1/200 - 1/2000.
ChIP Use at an assay dependent dilution. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.

Target

  • Relevance

    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • Alternative names

    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

Images

  • A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
  • All lanes : Anti-T7 tag® antibody (ab9138) at 1/25000 dilution

    Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
    Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
    Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg

References

This product has been referenced in:

See all 13 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
15 µg
Specification
293T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 08 2008

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T cells)
Total protein in input
1e+006 cells
Immuno-precipitation step
Protein A/G
Specification
293T cells

Abcam user community

Verified customer

Submitted Feb 08 2008

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