Key features and details
- Goat polyclonal to T7 tag®
- Suitable for: ELISA, WB, ICC, IHC-P
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-T7 tag® antibody
See all T7 tag® primary antibodies
DescriptionGoat polyclonal to T7 tag®
Tested applicationsSuitable for: ELISA, WB, ICC, IHC-Pmore details
Species reactivityReacts with: Species independent
T7 (MASMTGGQQMG) coupled to KLH
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab97964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.03 - 10 µg/ml. Coating: 2-10 ug/ml; Primary: 0.03-1 ug/ml|
|WB||Use a concentration of 0.03 - 1 µg/ml. Colorimetric: 0.1-1 ug/ml; Chemiluminescent: 0.03-1 ug/ml|
|ICC||Use a concentration of 0.5 - 5 µg/ml.|
|IHC-P||Use at an assay dependent dilution.|
RelevanceThe T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
All lanes : Anti-T7 tag® antibody (ab97964) at 0.4 µg/ml
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Developed using the ECL technique.
Exposure time: 30 seconds
ab97964 has not yet been referenced specifically in any publications.