Overview

  • Product name
    Anti-T7 tag® antibody (Agarose)
    See all T7 tag® primary antibodies
  • Description
    Goat polyclonal to T7 tag® (Agarose)
  • Host species
    Goat
  • Conjugation
    Agarose
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: ChIP
  • Immunogen

    Goats were immunized with

    MASMTGGQQMG

    (T7) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected.

  • General notes
    T7-TAG® is a trademark of EMD Biosciences, Inc.

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

Properties

Applications

Our Abpromise guarantee covers the use of ab1230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
  • Application notes
    Is unsuitable for ChIP.
  • Target

    • Relevance
      The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
    • Alternative names
      • 10 antibody
      • Major capsid protein 10A antibody
      • Major capsid protein antibody
      • Minor capsid protein 10B antibody
      • T7 epitope tag antibody
      see all

    References

    This product has been referenced in:
    • Piccinni E  et al. Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours. Acta Biochim Pol 58:529-34 (2011). IP . Read more (PubMed: 22068108) »
    • MacPherson MJ  et al. The CTCF insulator protein is posttranslationally modified by SUMO. Mol Cell Biol 29:714-25 (2009). IP . Read more (PubMed: 19029252) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    Indeed, we can confirm that Agarose-conjugated anti-T7 tag® antibodyab1230 can be used to purify (or pull-down) T7-tagged proteins, as it has been tested and is guaranteed to work in immunoprecipitation (IP).

    For a protocol you can refer to the publication by MacPherson who used thisproduct(see attached, and datasheet). For IP protocols in general our protocols website might be of interest for you: https://www.abcam.com/index.html?pageconfig=popular_protocols

    We currently have this product in stock. For further details on pricing and shipping time please contact our distributor in your country. By choosing your country in the top right corner of our website the contact details will be shown on each datasheet, e.g. for Australia it is currently

    Sapphire Bioscience Pty Ltd
    126 Cope Street
    Waterloo - NSW
    2017
    Australia
    tel +61296982022
    Fax +61296981022
    Website http://www.sapphirebioscience.com
    Email mailto:sales@sapphirebioscience.com
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Answer

    Thank you for contacting us for technical support with ab1230 on behalf of your customer. Unfortunately it is very hard for me to understand the customer's protocol and results as limited information was provided. Can you please explain what the different lanes are: -what is the positive control? -what is the difference between the two membrane images? (the lanes IP and Total cell lysate don't look the same at all between the two images, I am very confused) -what does "Rx" mean? I would like also to know more protocol information please: -how was the T7 antibody used in WB used: dilution, buffer, incubation time. Is there a positive control for this antibody? -what protease inhibitors were used in the RIPA buffer? -how long was the lysate mixed with the antibody/agarose slurry? -how were the agarose beads uncoupled from the antibody? -what blocking buffer was used: for the beads and the membrane? The more information you can provide the better it will be for me to assist your customer, thank you in advance,

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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