Key features and details
- Rabbit polyclonal to T7 tag® (Agarose)
- Suitable for: IP
- Reacts with: Species independent
- Conjugation: Agarose
- Isotype: IgG
Product nameAnti-T7 tag® antibody (Agarose)
See all T7 tag® primary antibodies
DescriptionRabbit polyclonal to T7 tag® (Agarose)
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Species independent
T7 tag (MASMTGGQQMG) conjugated to KLH.
General notesAmount: 0.1 mg antibody/200 µl of gel
Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 0.58% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab90665 was affinity purified using the peptide immobilized on solid support.
Our Abpromise guarantee covers the use of ab90665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab90665 has been referenced in 1 publication.
- Frigola J et al. Cdt1 stabilizes an open MCM ring for helicase loading. Nat Commun 8:15720 (2017). PubMed: 28643783