Product nameAnti-T7 tag® antibody (Alkaline Phosphatase)
See all T7 tag® primary antibodies
DescriptionRabbit polyclonal to T7 tag® (Alkaline Phosphatase)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Species independent
FormLyophilised:Reconstitute to 1 mL with ddH2O, vortex gently and allow material to reconstitute for 20 minutes, avoid exposure to heat. After product has been reconstituted, product should be stored at 2-8 C in the dark and be used within 6 months. For extended storage, add an equal volume of glycerol (ACS grade) for a final concentration of 50%, and store at -20 degrees Celsius. Prepare a working dilution daily.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.3% Tris, 0.88% Sodium chloride, 0.05% Magnesium chloride, 0.0016% Zinc chloride
Concentration information loading...
Our Abpromise guarantee covers the use of ab133151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/50000.|
|ELISA||1/5000 - 1/50000.|
RelevanceThe T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
ab133151 has not yet been referenced specifically in any publications.