Overview

  • Product name

    Anti-T7 tag® antibody (DyLight® 488)
    See all T7 tag® primary antibodies
  • Description

    Rabbit polyclonal to T7 tag® (DyLight® 488)
  • Host species

    Rabbit
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    T7 tag® with amino acid sequence MASMTGGQQMG.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1.4% Sodium phosphate, 0.88% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000.

Target

  • Relevance

    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • Alternative names

    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

Images

  • Spectral Characteristic
    Visible absorption maxima 488 nm
    Emission maximum 518 nm

References

This product has been referenced in:

  • Pye H  et al. Using antibody directed phototherapy to target oesophageal adenocarcinoma with heterogeneous HER2 expression. Oncotarget 9:22945-22959 (2018). Read more (PubMed: 29796164) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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