Overview

  • Product name

    Anti-T7 tag® antibody (Phycoerythrin)
    See all T7 tag® primary antibodies
  • Description

    Rabbit polyclonal to T7 tag® (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    T7 epitope tag conjugated to a carrier protein.

  • General notes

    Excitation maximum: 565>498 nm Emission maximum: 578 nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.013% Sodium azide
    Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab72563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab37409 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • Alternative names

    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

Images

  • Flow cytometry analysis of a recombinant (N-terminus T7-tag®) transmembrane cell surface receptor in CHO cells using ab72563 (yellow). Black - Mouse IgG1 Phycoerythrin isotype control.

References

ab72563 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. Your credit note ID is xxxx I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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