Overview

  • Product name

    Anti-TAB1 antibody [EPR635Y]
    See all TAB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR635Y] to TAB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TAB1 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: MCF-7, Mouse brain, Rat brain, and Human brain lysates; IHC-P: human tonsil, human lung carcinoma, mouse stomach, and rat kidney tissues; ICC/IF: HeLa cells; FC: HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB 1/1000 - 1/2000. Predicted molecular weight: 55 kDa.
Flow Cyt 1/20.

For unpurified use at 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250.

For unpurified use at 1/50 - 1/100.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Contains 1 PP2C-like domain.
    • Post-translational
      modifications

      Monoubiquitinated. Deubiquitinated by Y.enterocolitica YopP.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2310012M03Rik antibody
      • 3'-Tab1 antibody
      • MAP3K7IP 1 antibody
      • MAP3K7IP1 antibody
      • MGC57664 antibody
      • Mitogen activated protein kinase kinase kinase 7 interacting protein 1 antibody
      • Mitogen-activated protein kinase kinase kinase 7-interacting protein 1 antibody
      • TAB 1 antibody
      • TAB1 antibody
      • TAB1_HUMAN antibody
      • TAK1 binding protein 1 antibody
      • TAK1-binding protein 1 antibody
      • TGF beta activated kinase 1 binding protein 1 antibody
      • TGF-beta activated kinase 1/MAP3K7 binding protein 1 antibody
      • TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 antibody
      • TGF-beta-activated kinase 1-binding protein 1 antibody
      • Transforming growth factor beta activated kinase binding protein 1 antibody
      see all

    Images

    • All lanes : Anti-TAB1 antibody [EPR635Y] (ab76412) at 1/1000 dilution (Purified)

      Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse brain lysates
      Lane 3 : Rat brain lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling TAB1 with Purified ab76412 at 1/250 dilution (0.71 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TAB1 with purified ab76412 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TAB1 with Purifiedab76412 at 1/250 dilution (0.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling TAB1 with purified ab76412 at 1/250 dilution (0.71 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TAB1 with purified ab76412 at 1/250 dilution (0.71 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Anti-TAB1 antibody [EPR635Y] (ab76412) at 1/1000 dilution (Purified) + Human brain lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa

    • Anti-TAB1 antibody [EPR635Y] (ab76412) at 1/2000 dilution (unpurified) + MCF-7 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa

    • Overlay histogram showing HeLa cells stained with unpurified ab76412 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76412, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunohistochemical staining of TAB1 in paraffin embedded human tonsils using unpurified ab76412 at a 1/100 dilution.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Yang YQ  et al. Triptriolide Alleviates Lipopolysaccharide-Induced Liver Injury by Nrf2 and NF-?B Signaling Pathways. Front Pharmacol 9:999 (2018). Read more (PubMed: 30210350) »
    • Huang HL  et al. Targeting of TGF-ß-activated protein kinase 1 inhibits chemokine (C-C motif) receptor 7 expression, tumor growth and metastasis in breast cancer. Oncotarget 6:995-1007 (2015). Read more (PubMed: 25557171) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Total protein in input
    500 µg
    Specification
    MEFs
    Immuno-precipitation step
    Protein A/G

    Abcam user community

    Verified customer

    Submitted Aug 02 2010

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