Anti-TAB2 antibody (ab223242)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TAB2 antibody
See all TAB2 primary antibodies -
Description
Goat polyclonal to TAB2 -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human TAB2 aa 161-173 (internal sequence) (Cysteine residue).
Sequence:C-SKGTSSLSQQTPR
Database link: Q9NYJ8 -
Positive control
- IHC: Human colon tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223242 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab223242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Adapter linking MAP3K7/TAK1 and TRAF6. Promotes MAP3K7 activation in the IL1 signaling pathway. The binding of 'Lys-63'-linked polyubiquitin chains to TAB2 promotes autophosphorylation of MAP3K7 at 'Thr-187'. Involved in heart development. -
Tissue specificity
Widely expressed. In the embryo, expressed in the ventricular trabeculae, endothelial cells of the conotruncal cushions of the outflow tract and in the endothelial cells lining the developing aortic valves. -
Involvement in disease
Defects in TAB2 are the cause of congenital heart disease non-syndromic type 2 (CHTD2) [MIM:612863]. It is a disease characterized by congenital developmental abnormalities involving structures of the heart. Clinical features include left ventricular outflow tract obstruction, subaortic stenosis, residual aortic regurgitation, atrial fibrillation, bicuspid aortic valve and aortic dilation. Note=A chromosomal aberration involving TAB2 has been found in a family with congenital heart disease. Translocation t(2;6)(q21;q25). -
Sequence similarities
Contains 1 CUE domain.
Contains 1 RanBP2-type zinc finger. -
Post-translational
modificationsUbiquitinated; following IL1 stimulation or TRAF6 overexpression. Ubiquitination involves RBCK1 leading to proteasomal degradation.
Phosphorylated. -
Cellular localization
Membrane. Cytoplasm > cytosol. Following IL1 stimulation, translocation occurs from the membrane to cytosol. - Information by UniProt
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Database links
- Entrez Gene: 23118 Human
- Entrez Gene: 68652 Mouse
- Entrez Gene: 308267 Rat
- Omim: 605101 Human
- SwissProt: Q9NYJ8 Human
- SwissProt: Q99K90 Mouse
- SwissProt: Q5U303 Rat
- Unigene: 269775 Human
see all -
Alternative names
- CHTD2 antibody
- FLJ21885 antibody
- KIAA0733 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAB2 antibody (ab223242)Paraffin-embedded human colon tissue stained for TAB2 with ab223242 at 3.8 μg/ml in immunohistochemical analysis, followed by AP-staining.
Protocols
Datasheets and documents
References
ab223242 has not yet been referenced specifically in any publications.
