Anti-TAB3 antibody (ab85655)
Key features and details
- Rabbit polyclonal to TAB3
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TAB3 antibody
See all TAB3 primary antibodies -
Description
Rabbit polyclonal to TAB3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese hamster, Orangutan, Elephant -
Immunogen
Synthetic peptide, corresponding to amino acids 600-650 of Human TAB3
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 79 kDa.
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IP |
Use at 5-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 79 kDa. |
IP
Use at 5-10 µg/mg of lysate. |
Target
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Function
Adapter linking MAP3K7/TAK1 and TRAF6 or TRAF2. Mediator of MAP3K7 activation, respectively in the IL1 and TNF signaling pathways. Plays a role in activation of NF-kappa-B and AP1 transcription factor. Isoform 2 may be an oncogenic factor. -
Tissue specificity
Widely expressed. Constitutively overexpressed in certain tumor tissues. Isoform 1 is a major transcript while isoform 2 is a minor transcript. -
Sequence similarities
Contains 1 CUE domain.
Contains 1 RanBP2-type zinc finger. -
Post-translational
modificationsUbiquitinated; following IL1 stimulation or TRAF6 overexpression. - Information by UniProt
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Database links
- Entrez Gene: 257397 Human
- Entrez Gene: 66724 Mouse
- Omim: 300480 Human
- SwissProt: Q8N5C8 Human
- SwissProt: Q571K4 Mouse
- Unigene: 188256 Human
- Unigene: 119646 Mouse
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Alternative names
- MAP3K7IP 3 antibody
- Mitogen-activated protein kinase kinase kinase 7-interacting protein 3 antibody
- NAP1 antibody
see all
Images
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All lanes : Anti-TAB3 antibody (ab85655) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 79 kDa
Exposure time: 30 seconds -
1mg whole cell lysate from HeLa cells was immunoprecipitated using 10µg ab85655.
20% of the immunoprecipitate was loaded in each lane, and probed with ab85655 at 1µg/ml (lane 1) or with a control IgG (lane 2).
Detection: chemoluminescence, with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab85655 has been referenced in 7 publications.
- Du K et al. MicroRNA-16 inhibits the lipopolysaccharide-induced inflammatory response in nucleus pulposus cells of the intervertebral disc by targeting TAB3. Arch Med Sci 17:500-513 (2021). PubMed: 33747285
- Zhang J et al. MiR-103a-3p aggravates renal cell carcinoma by targeting TMEM33. Am J Transl Res 13:12694-12703 (2021). PubMed: 34956484
- Zhou Q et al. USP15 potentiates NF-κB activation by differentially stabilizing TAB2 and TAB3. FEBS J 287:3165-3183 (2020). PubMed: 31903660
- Sheng C et al. Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing. Nat Commun 9:4381 (2018). PubMed: 30348973
- Lin Y & Luo Z NLRP6 facilitates the interaction between TAB2/3 and TRIM38 in rheumatoid arthritis fibroblast-like synoviocytes. FEBS Lett 591:1141-1149 (2017). WB ; Human . PubMed: 28295271
- Zhao N et al. MicroRNA-26b suppresses the NF-?B signaling and enhances the chemosensitivity of hepatocellular carcinoma cells by targeting TAK1 and TAB3. Mol Cancer 13:35 (2014). WB ; Human . PubMed: 24565101
- Gardner A et al. The critical role of TAK1 in accentuated epithelial to mesenchymal transition in obliterative bronchiolitis after lung transplantation. Am J Pathol 180:2293-308 (2012). PubMed: 22525462