Key features and details
- Rabbit polyclonal to TACC1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TACC1 antibody
DescriptionRabbit polyclonal to TACC1
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- Human placenta tissue; MCF7 cells; K562 cell extract.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, 49% PBS
PBS (without Mg2+, Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 88 kDa.|
|ICC/IF||1/100 - 1/500.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionLikely involved in the processes that promote cell division prior to the formation of differentiated tissues.
Tissue specificityIsoform 1, isoform 3 and isoform 5 are ubiquitous. Isoform 2 is strongly expressed in the brain, weakly detectable in lung and colon, and overexpressed in gastric cancer. Isoform 4 is not detected in normal tissues, but strong expression was found in gastric cancer tissues. Down-regulated in a subset of cases of breast cancer.
Sequence similaritiesBelongs to the TACC family.
Contains 2 SPAZ (Ser/Pro-rich AZU-1) domains.
Developmental stageExpressed at high level during early embryogenesis.
modificationsIsoform 1 is heavily phosphorylated; isoform 6 is not. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. Nucleus during interphase. Weakly concentrated at centrosomes during mitosis and colocalizes with AURKC at the midbody during cytokinesis.
- Information by UniProt
- DKFZp686K18126 antibody
- Ga 55 antibody
- Ga55 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human plancenta tissue labeling TACC1 with ab211478 at 10µg/mL.
Immunofluorescent analysis of MCF7 cells labeling TACC1 with ab211478 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
All lanes : Anti-TACC1 antibody (ab211478) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : K562 cell extract with synthesized peptide
Predicted band size: 88 kDa
ab211478 has not yet been referenced specifically in any publications.