Product nameAnti-TACC3 antibody
See all TACC3 primary antibodies
DescriptionRabbit polyclonal to TACC3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TACC3 aa 425-475. The exact sequence is proprietary. NP_006333.1
Database link: Q9Y6A5
- WB:HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245455 was affinity purified using an epitope specific to TACC3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 90 kDa.|
FunctionPlays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). May be involved in the control of cell growth and differentiation. May contribute to cancer.
Sequence similaritiesBelongs to the TACC family.
- Information by UniProt
- ERIC 1 antibody
- ERIC-1 antibody
- ERIC1 antibody
All lanes : Anti-TACC3 antibody (ab245455) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 90 kDa
Exposure time: 30 seconds
TACC3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245455 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245455 at 0.4 µg/ml.
Lane 1: ab245455 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab245455 has not yet been referenced specifically in any publications.