Overview

  • Product name

  • Description

    Rabbit polyclonal to TACC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TACC3 aa 425-475. The exact sequence is proprietary. NP_006333.1
    Database link: Q9Y6A5

  • Positive control

    • WB:HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245455 was affinity purified using an epitope specific to TACC3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 90 kDa.

Target

  • Function

    Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). May be involved in the control of cell growth and differentiation. May contribute to cancer.
  • Sequence similarities

    Belongs to the TACC family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ERIC 1 antibody
    • ERIC-1 antibody
    • ERIC1 antibody
    • MGC117382 antibody
    • MGC133242 antibody
    • OTTHUMP00000113796 antibody
    • TACC3 antibody
    • TACC3_HUMAN antibody
    • Transforming acidic coiled coil containing protein 3 antibody
    • Transforming acidic coiled-coil-containing protein 3 antibody
    see all

Images

  • All lanes : Anti-TACC3 antibody (ab245455) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 90 kDa


    Exposure time: 30 seconds
  • TACC3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245455 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245455 at 0.4 µg/ml.

    Lane 1: ab245455 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab245455 has not yet been referenced specifically in any publications.

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