Overview

  • Product name

    Anti-TACC3 antibody [EPR7756]
    See all TACC3 primary antibodies
  • Description

    Rabbit monoclonal [EPR7756] to TACC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TACC3 aa 50-150. The exact sequence is proprietary.
    Database link: Q9Y6A5

  • Positive control

    • WB: Human lymph node tissue lysate. Mouse and rat testis tissue lysate. HT-29 and HeLa whole cell lysate (ab150035). IHC-P: Human testis and tonsil tissues. ICC/IF: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7756
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 90 kDa.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100.

ICC/IF 1/200 - 1/400.

For unpurified use at 1/50 - 1/100.

Target

  • Function

    Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). May be involved in the control of cell growth and differentiation. May contribute to cancer.
  • Sequence similarities

    Belongs to the TACC family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ERIC 1 antibody
    • ERIC-1 antibody
    • ERIC1 antibody
    • MGC117382 antibody
    • MGC133242 antibody
    • OTTHUMP00000113796 antibody
    • TACC3 antibody
    • TACC3_HUMAN antibody
    • Transforming acidic coiled coil containing protein 3 antibody
    • Transforming acidic coiled-coil-containing protein 3 antibody
    see all

Images

  • Anti-TACC3 antibody [EPR7756] (ab134154) at 1/2000 dilution (purified) + HeLa cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TACC3 with purified ab134154 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/200) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Anti-TACC3 antibody [EPR7756] (ab134154) at 1/10000 dilution (purified) + Human lymph node tissue lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/10000 dilution (purified)

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/1000 dilution (unpurified)

    Lane 1 : Human lymph node tissue lysate
    Lane 2 : HT-29 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 90 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TACC3 with purified ab134154 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling TACC3 with unpurified ab134154 at 1/50 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TACC3 with unpurified ab134154 at 1/50 dilution.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TACC3 with unpurified ab134154 at 1/50 dilution.

References

This product has been referenced in:

  • Zhao C  et al. Downregulation of TACC3 inhibits tumor growth and migration in osteosarcoma cells through regulation of the NF-?B signaling pathway. Oncol Lett 15:6881-6886 (2018). Read more (PubMed: 29725420) »
  • Wang Y  et al. Long non-coding RNA lnc-PCTST predicts prognosis through inhibiting progression of pancreatic cancer by downregulation of TACC-3. Int J Cancer N/A:N/A (2018). Read more (PubMed: 29978472) »
See all 8 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry, which was passed along to me from my colleague Chris in Marketing.

I BLASTed the immunogen sequence for ab56595 against mouse and the homology was only 66%. Generally we say that anything with >85% sequence homology would be expected to cross-react, so this is quite low. Plus, since it's a mouse monoclonal, you may run into high background staining with ab56595 on mouse tissues.

Instead, I would recommend ab134154, a rabbit monoclonal anti-mouse TACC3 antibody.

https://www.abcam.com/TACC3-antibody-EPR7756-ab134154.html

I hope this information helps. Please contact us with any other questions.

Read More

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