Recombinant Anti-TACC3 antibody [EPR7756] (ab134154)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7756] to TACC3
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TACC3 antibody [EPR7756]
See all TACC3 primary antibodies -
Description
Rabbit monoclonal [EPR7756] to TACC3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TACC3 aa 50-150. The exact sequence is proprietary.
Database link: Q9Y6A5 -
Positive control
- WB: Human lymph node tissue lysate. Mouse and rat testis tissue lysate. HEK293T, HCT116, HT-29 and HeLa whole cell lysate (ab150035). IHC-P: Human testis and tonsil tissues. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7756 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 90 kDa.
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IHC-P |
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. |
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ICC/IF |
1/200 - 1/400.
For unpurified use at 1/50 - 1/100. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 90 kDa. |
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. |
ICC/IF
1/200 - 1/400. For unpurified use at 1/50 - 1/100. |
Target
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Function
Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). May be involved in the control of cell growth and differentiation. May contribute to cancer. -
Sequence similarities
Belongs to the TACC family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10460 Human
- Entrez Gene: 21335 Mouse
- Entrez Gene: 360962 Rat
- Omim: 605303 Human
- SwissProt: Q9Y6A5 Human
- SwissProt: Q9JJ11 Mouse
- Unigene: 104019 Human
- Unigene: 379024 Mouse
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Alternative names
- ERIC 1 antibody
- ERIC-1 antibody
- ERIC1 antibody
see all
Images
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All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TACC3 knockout HEK293T cell lysate
Lane 3 : HCT116 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 90 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab134154 observed at 125 kDa. Red - loading control ab8245 observed at 36 kDa.
ab134154 Anti-TACC3 antibody [EPR7756] was shown to specifically react with TACC3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266316 (knockout cell lysate ab257723) was used. Wild-type and TACC3 knockout samples were subjected to SDS-PAGE. ab134154 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TACC3 with purified ab134154 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/200) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TACC3 with purified ab134154 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.
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Anti-TACC3 antibody [EPR7756] (ab134154) at 1/2000 dilution (purified) + HeLa cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-TACC3 antibody [EPR7756] (ab134154) at 1/10000 dilution (purified) + Human lymph node tissue lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/10000 dilution (purified)
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/1000 dilution (unpurified)
Lane 1 : Human lymph node tissue lysate
Lane 2 : HT-29 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 90 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling TACC3 with unpurified ab134154 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TACC3 with unpurified ab134154 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TACC3 with unpurified ab134154 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab134154 has been referenced in 19 publications.
- Wang X et al. Comprehensive assessment of cellular senescence in the tumor microenvironment. Brief Bioinform 23:N/A (2022). PubMed: 35419596
- Abbassi L et al. Epidermal growth factor receptor signaling uncouples germ cells from the somatic follicular compartment at ovulation. Nat Commun 12:1438 (2021). PubMed: 33664246
- Zhang SW et al. Co-expression of SOX2 and HR-HPV RISH predicts poor prognosis in small cell neuroendocrine carcinoma of the uterine cervix. BMC Cancer 21:332 (2021). PubMed: 33789601
- Wen YL et al. Transforming acidic coiled-coil protein-3: a novel marker for differential diagnosis and prognosis prediction in endocervical adenocarcinoma. Mol Med 27:60 (2021). PubMed: 34134633
- Wang X et al. Loss of acentriolar MTOCs disrupts spindle pole Aurora A and assembly of the liquid-like meiotic spindle domain in oocytes. J Cell Sci 134:N/A (2021). PubMed: 34152366