Overview

  • Product name

    TACE Inhibitor Assay Kit
  • Detection method

    Fluorescent
  • Assay type

    Quantitative
  • Assay time

    0h 20m
  • Product overview

    In Abcam’s TACE inhibitor screening Kit, TACE (tumor necrosis factor-a-converting enzyme) hydrolyzes the specific FRET substrate to release the quenched fluorescent group, which can be detected fluorometrically at Ex/Em = 318/449 nm. In the presence of the potent TACE inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of TACE inhibitors and can be modified to check the relative TACE activity. Inhibitor Control GM6001 is included to compare the efficacy of test inhibitors.

  • Notes

    The TACE (tumor necrosis factor-a-converting enzyme), also called ADAM metallopeptidase domain 17 (ADAM17), is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. TACE is believed to be involved in the processing of tumor necrosis factor alpha (TNF-a) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. This process, which is also known as 'shedding', involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-a), and is of known physiological importance.

  • Platform

    Microplate reader

Properties

Images

  • TACE activity with increasing GM6001 inhibition. Produced following kit protocol.

  • Fluorescence of TACE enzyme control and background. Produced following kit protocol.

Protocols

References

ab155889 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

The TACE enzyme in the ab155889 assay is human recombinant protein, 20ug per vial.

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Answer

The protocol for the ab155889 TACE Inhibitor Screening Kit has been designed for working with the assay buffer. For it to work with the cell culture media, some individual further optimization may be required.

I am sorry that as we have not tested this type of sample ourselves, it is regrettably not possible to confirm for sure whether it will be successful or not. The one suggestion we can provide is to try diluting whatever sample you plan to use with the kit assay buffer so that all the ingredients required for the reaction to occur are present in the well.

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