TACE Inhibitor Assay Kit (ab155889)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
Overview
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Product name
TACE Inhibitor Assay Kit -
Detection method
Fluorescent -
Assay type
Quantitative -
Assay time
0h 20m -
Product overview
In Abcam’s TACE inhibitor screening Kit, TACE (tumor necrosis factor-α-converting enzyme) hydrolyzes the specific FRET substrate to release the quenched fluorescent group, which can be detected fluorometrically at Ex/Em = 318/449 nm. In the presence of the potent TACE inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of TACE inhibitors and can be modified to check the relative TACE activity. Inhibitor Control GM6001 is included to compare the efficacy of test inhibitors.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K366 TACE Inhibitor Screening Assay Kit ( Fluorometric). K366-100 is the same size as the 100 test size of ab155889.
The TACE (tumor necrosis factor-α-converting enzyme), also called ADAM metallopeptidase domain 17 (ADAM17), is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. TACE is believed to be involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. This process, which is also known as 'shedding', involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-α), and is of known physiological importance.
The standard is affinity purified.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests TACE Assay Buffer 1 x 25ml TACE Enzyme 1 vial TACE Inhibitor Control 1 x 20µl TACE Substrate 1 x 0.2ml -
Research areas
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155889 has not yet been referenced specifically in any publications.