Overview

  • Product name

  • Description

    Rabbit polyclonal to TADA3L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Giant Panda
  • Immunogen

    Synthetic peptide within Human TADA3L aa 325-375. The exact sequence is proprietary.
    Database link: O75528

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance

      TADA3L functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. TADA3L is also known as a coactivator for p53/TP53-dependent transcriptional activation. The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • ADA3 antibody
      • ADA3 homolog antibody
      • ADA3-like protein antibody
      • FLJ20221 antibody
      • FLJ21329 antibody
      • hADA3 antibody
      • Transcriptional adaptor 3 (NGG1 homolog yeast) like antibody
      • Transcriptional adaptor 3 like antibody
      see all

    Images

    • TADA3L was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226306 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226306 at 0.4 µg/ml.

      Lane 1: ab226306 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 75 seconds.

    References

    ab226306 has not yet been referenced specifically in any publications.

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