Product nameAnti-TADA3L antibody
See all TADA3L primary antibodies
DescriptionRabbit polyclonal to TADA3L
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
- IHC-P: Human vagina tissue. WB: RT4, NIH/3T3, NBT-II and U-251 MG cell lysate. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceTADA3L functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. TADA3L is also known as a coactivator for p53/TP53-dependent transcriptional activation. The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3.
- ADA3 antibody
- ADA3 homolog antibody
- ADA3-like protein antibody
Paraffin-embedded human vagina tissue stained for TADA3L using ab254869 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-TADA3L antibody (ab254869) at 0.4 µg/ml
Lane 1 : NIH/3T3 cell lysate
Lane 2 : NBT-II cell lysate
Lanes 2-3 : Anti-TADA3L antibody (ab254869) at 0.4 µg/ml
Lane 1 : DNA Ladder
Lane 2 : RT4 cell lysate
Lane 3 : U-251 MG cell lysate
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling TADA3L using ab254869 at 4µg/ml (green) in ICC/IF.
ab254869 has not yet been referenced specifically in any publications.